Medical Laboratory Testing Guidelines in Toronto Healthcare Facilities

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This document outlines the specific tube colors, volumes, and labeling instructions for serology and HLA testing at Toronto Medical Laboratories (TML) and the Hospital for Sick Children. It includes details on sample collection, handling, and delivery procedures for different patient categories. The guidelines emphasize the importance of accurate labeling and proper sample handling to ensure precise test results.


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  1. Toronto Serology (TML does NAT testing for province) Colour # of tubes 2 Pink (EDTA) or Lavender (EDTA) NAT does not require extra tube WNV (May October) extra tube not required Red 2 Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials (if space) Send to: Toronto Medical Laboratories (TML) 600 University Ave, Room 1470 (Serology) Toronto ON M5G 1X5 Who delivers? Co-op Taxi 416-504-2667 Account 26261 Where does delivery service pick up? Arranged at time of call January 3, 2020

  2. Toronto HLA Colour # of tubes Yellow (ACD) 10 Pink (EDTA) or Lavender (EDTA) 2 10 Yellow (ACD) Provincial Samples DO NOT DRAW until instructed by PRC Send to PRC when instructed (address below) Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - Date of draw - Time of draw - OTDC Initials (if space) Send to: Local Samples Provincial Samples (PRC): TGLN Provincial Resource Centre 483 Bay St 4th Floor SOUTH Toronto ON M5G 2C9 200 Elizabeth Street 11E-444 Toronto ON M5G 2C4 Who delivers? Co-op Taxi 416-504-2667 Account 26261 Where does delivery service pick up? Arranged at time of call January 3, 2020

  3. Hospital for Sick Childrens Serology Colour Infant (< 10kg) Child (10.1 50kg) Adolescent (> 50kg) Pink (EDTA) or Lavender (EDTA) 1-5 cc 3-5 cc 5 cc Red 3-5 cc 6-10 cc 10-14 cc Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials (if space) Special considerations: If child (a) <18 months or (b) breastfed in past 12 months: - Need maternal serology - 2 RED - 1 EDTA (PINK/LAVENDER) Send to: Toronto Medical Laboratories 600 University Ave, Room 1470 (Serology) Toronto ON M5G 1X5 Who delivers? Co-op Taxi 416-504-2667 Account 26261 Where does delivery service pick up? Arranged at time of call January 3, 2020

  4. Hospital for Sick Childrens HLA Colour Infant (< 10kg) Child (10.1 50kg) Adolescent (> 50kg) Tissue Typing 3-5 cc 6-10 cc 10 cc Pink (EDTA) or Lavender (EDTA) Infant/Child (< 4 years) Child Child Adolescent (>14 years) Colour (4-10 years) (10-14 years) Yellow ACD (Cross Matching) Minimum 1 tube (approx 8.5-10 cc) 20-40 cc 40-60 cc 60-100 cc Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - OTDC Initials (if space) - Date of draw - Time of draw Provincial Samples DO NOT DRAW until instructed by PRC Send to: 200 Elizabeth Street 11E-444 Toronto ON M5G 2C4 Who delivers? Co-op Taxi 416-504-2667 Account 26261 Where does delivery service pick up? Arranged at time of call January 3, 2020

  5. Near North and areas Serology Colour # of tubes 2 Pink (EDTA) or Lavender (EDTA) WNV (May-October) extra tube not required NAT does not require extra tube Red 2 Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials (if space) Send to: Toronto Medical Laboratories 600 University Ave, Room 1470 (Serology) Toronto ON M5G 1X5 Who delivers? Apple Courier 1-888-942-7753 Account 1170 Timmins/Sault/North Bay: Air Canada Cargo (if needed) Where does delivery service pick up? Arranged at time of call January 3, 2020

  6. Near North and areas HLA Colour # of tubes Yellow (ACD) - Local 10 Pink or Lavender 2 Provincial Samples 10 Yellow (ACD) **Timmins/Sault/North Bay to always send PRC blood samples. DO NOT DRAW until instructed by PRC Send to PRC when instructed. Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - Date of draw - Time of draw - OTDC Initials (if space) Send to: 200 Elizabeth Street 11E-444 Toronto ON M5G 2C4 Provincial Samples (PRC): Provincial Resource Centre 483 Bay St 4th Floor SOUTH Toronto ON M5G 2C9 Who delivers? Apple Courier 1-888-942-7753 x1 Account 1170 Sault/ Timmins / North Bay: Air Canada Cargo (if needed) Where does delivery service pick up? Arranged at time of call January 3, 2020

  7. Hamilton/Niagara/Kitchener and area Serology Colour # of tubes 2 Pink (EDTA) or Lavender (EDTA) WNV (May-October) extra tube not required NAT does not require extra tube Red 2 Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials (if space) Send to: Toronto Medical Laboratories 600 University Ave, Room 1470 (Serology) Toronto ON M5G 1X5 Who delivers? Apple Courier 1-888-942-7753 x1 Account 1170 Where does delivery service pick up? Meet in ICU January 3, 2020

  8. Hamilton/Niagara/Kitchener and area HLA Colour # of tubes Yellow (ACD) - Local 10 Pink or Lavender 2 10 Yellow (ACD) Provincial Samples DO NOT DRAW until instructed by PRC Send to PRC when instructed (address below) Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - Date of draw - Time of draw - OTDC Initials (if space) Send to: Local Samples: Provincial Samples: Provincial Resource Centre 483 Bay St 4th Floor SOUTH Toronto ON M5G 2C9 St Joseph Healthcare (Emerg Desk) 50 Charlton Ave East Hamilton ON L8N 4A6 Who delivers? Hamilton Cab 905-777-7777 Account Trillium Gift of Life Apple Courier 1-888-942-7753 Account 1170 Where does delivery service pick up? Front entrance or Emergency Meet in ICU (send with serology) January 3, 2020

  9. London Serology Colour # of tubes 2 Pink (EDTA) or Lavender (EDTA) +1 for WNV (May-October) Red 1 Gold (SST) 3 Include on label: Ensure tube lot # and expiry date are visible. - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials (if space) Send to: Samples at LHSC: Drop off at Core Lab of internal hospital where donor is located. Core Lab will redistribute serology and HLA tubes to appropriate lab. Samples from outside London: London Health Sciences Centre Core Lab University Hospital 339 Windermere Road London ON N6A 5A5 Who delivers? OTDC/Porter Apple Courier 1-888-942-7753 Account 1170 Where does delivery service pick up? January 3, 2020 Drop off at Core Lab Arranged at courier arrival

  10. London HLA Colour # of tubes Yellow (ACD) - Local 10 Pink (EDTA) or Lavender (EDTA) 6 10 Yellow (ACD) Provincial Samples DO NOT DRAW until instructed by PRC Send to PRC when instructed (address below) Include on Label: - TGLN # - DOB Ensure tube lot # and expiry date are visible. - OTDC Initials (if space) - Date of draw - Time of draw Send to: Samples at LHSC: Drop off at Core Lab of internal hospital where donor is located. Core Lab will redistribute serology and HLA tubes to appropriate lab. All Samples (outside LHSC): London Health Sciences Centre Core Lab-University Hospital 339 Windermere Road London ON N6A 5A5 Provincial Samples: Provincial Resource Centre 483 Bay St 4th Floor SOUTH Toronto ON M5G 2C9 Who delivers? OTDC/Porter Apple Courier 1-888-942-7753 Account 1170 Drop off? Dropped off at Core Lab Arranged at courier arrival January 3, 2020

  11. Kingston and area Serology Colour KGH, Brockville, Quinte # of tubes Peterborough # of tubes Red 3 2 Lavender (EDTA) Pink (EDTA) or Not required 2 Include on Label: Ensure tube lot # and expiry date are visible. - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials Send to: Kingston, Brockville, Quinte: Biochemistry Lab Children s Hospital of Eastern Ontario (CHEO) 401 Smyth Road, 3rd Floor Rm 3030 Ottawa ON K1H 8L1 Peterborough: Toronto Medical Labs (Serology) 600 University Ave, Room 1470 Toronto ON M5G 1X5 Who delivers? Apple Courier 1-888-942-7753 Account 1170 Where does delivery service pick up? Pick up directly in ICU January 3, 2020

  12. Kingston and area WNV Colour KGH, Brockville, Quinte # of tubes Peterborough # of tubes 2 WNV (May-October only) (Off season pink/lavender tube is not required) Lavender (EDTA) Pink (EDTA) or No extra tubes required for WNV Include on Label: Ensure tube lot # and expiry date are visible. - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials Send to: Kingston, Brockville, Quinte: Microbiology Ottawa General Campus 501 Smyth Road. 3rd floor Rm 3608 Ottawa, ON K1H 8L6 Peterborough: Toronto Medical Labs (Serology) 600 University Ave, Room 1470 Toronto ON M5G 1X5 Who delivers? Apple Courier 1-888-942-7753 Account 1170 Where does delivery service pick up? Pick up directly in ICU August 4, 2020

  13. Kingston and area HLA Colour # of tubes 10 Kingston, Quinte 6 tubes required for Brockville Yellow (ACD) - Local 0 Kingston, Quinte 2 Peterborough, Brockville Pink or Lavender 10 Yellow (ACD) Provincial Samples DO NOT DRAW until instructed by PRC Send to PRC when instructed (address below) Include on Label: - TGLN # - Date of draw - DOB - Time of draw - OTDC initials Ensure lot # and expiry date are visible. Send to: Kingston, Quinte: KGH Immunology Lab 76 Stuart Street Kingston ON 613-549-6666 x4188 Brockville: Peterborough: 200 Elizabeth Street 11E-444 Toronto ON M5G 2C4 Provincial Samples: Provincial Resource Centre 483 Bay St 4th Floor SOUTH Toronto ON M5G 2C9 Ottawa General Hospital Tissue Typing DNA Lab 501 Smyth Rd, Rm 4155 Ottawa ON K1H 8L6 Delivery to STAT Specimen Receiving Rm3608 Who delivers? Kingston: OTDC Quinte, Brockville, Peterborough, Provincial Samples: Apple Courier 1-888-942-7753 Account 1170 Call blood bank (x4188) after hours to notify HLA tech M-F 0800-1600: HLA Lab (Douglas 4) Off-hours: Blood Bank (Douglas 1) Drop off? Arranged at courier arrival August 4, 2020

  14. Ottawa Serology Colour # of tubes Red 3 Include on Label: Ensure tube lot # and expiry date are visible. - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials (if space) Send to: Biochemistry Lab Children s Hospital of Eastern Ontario (CHEO) 401 Smyth Road, 3rd Floor Rm 3030 Ottawa ON K1H 8L1 Who delivers? The Ottawa Hospital (General Campus ONLY) OTDC to drop off at lab All other Ottawa region hospitals Apple Courier 1-888-942-7753 Account 1170 3rd floor sample reception Where to hand off? ICU main desk January 3, 2020

  15. Ottawa WNV Colour # of tubes 2 WNV (May 1-October 31) (Off season pink/lavender tube is not required) Pink (EDTA) or Lavender (EDTA) Include on Label: Ensure tube lot # and expiry date are visible. - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials (if space) Send to: Microbiology lab General Campus 501 Smyth Road. 3rd floor Rm 3608 Ottawa, ON K1H 8L6 Who delivers? The Ottawa Hospital (General Campus ONLY) OTDC to drop off at lab All other Ottawa region hospitals Apple Courier 1-888-942-7753 Account 1170 3rd floor main lab desk (it will be transported to appropriate labs from there) Where to hand off? ICU main desk August 4, 2020

  16. Ottawa HLA Colour # of tubes Yellow (ACD) - Local 6 Pink (EDTA) or Lavender (EDTA) 2 10 Yellow (ACD) Provincial Samples DO NOT DRAW until instructed by PRC Send to PRC when instructed (address below) Include on Label: - TGLN # - Date of draw Ensure tube lot # and expiry date are visible. - DOB - Time of draw - OTDC initials (if space) Send to: Ottawa General Hospital Tissue Typing DNA Lab 501 Smyth Rd, Rm 4155 Ottawa ON K1H 8L6 Delivery to STAT Specimen Receiving Rm 3608 Provincial Samples: TGLN Provincial Resource Centre 483 Bay St 4th Floor SOUTH Toronto ON M5G 2C9 Who delivers? The Ottawa Hospital (General Campus ONLY) OTDC to drop off at lab All other Ottawa region hospitals Apple Courier 1-888-942-7753 Account 1170 3rd floor main lab desk (it will be transported to appropriate labs from there) Where to hand off? ICU main desk August 4, 2020

  17. Sudbury Serology Colour # of tubes 2 Pink (EDTA) or Lavender (EDTA) WNV (May-October) extra tube not required NAT does not require extra tube Red 2 Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials (if space) Send to: Toronto Medical Laboratories 600 University Ave, Room 1470 (Serology) Toronto ON M5G 1X5 Who delivers? Apple Courier 1-888-942-7753 Account 1170 Where does delivery service pick up? Security Desk at Main Lobby (HSN) January 3, 2020

  18. Sudbury HLA Colour # of tubes 20 Yellow (ACD) London and Provincial (10 to LHSC plus 10 to Provincial Samples) Pink (EDTA) or Lavender (EDTA) 8 (to LHSC) Red London 1 (to LHSC) Gold (SST) - London 1 (to LHSC) Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - OTDC Initials (if space) - Date of draw - Time of draw Send to: London Health Sciences Centre HLA Lab University Hospital 339 Windermere Road London ON N6A 5A5 Provincial Samples: Provincial Resource Centre 483 Bay St 4th Floor SOUTH Toronto ON M5G 2C9 Who delivers? Apple Courier 1-888-942-7753 Account 1170 Where does delivery service pick up? Security Desk at Main Lobby (HSN) January 3, 2020

  19. Thunder Bay Serology Colour # of tubes Pink (EDTA) or Lavender (EDTA) 2 2 Red WNV (May-October) extra tube not required NAT does not require extra tube Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials (if space) Send to: Toronto Medical Laboratories 600 University Ave, Room 1470 (Serology) Toronto ON M5G 1X5 Who delivers? Taxi (to Air Canada Cargo) 807-577-1193 Account TRILLIUM Where does delivery service pick up? Arranged at time of call OTDC may also drop off directly at Air Canada Cargo January 3, 2020

  20. Thunder Bay HLA Colour # of tubes 20 Yellow (ACD) (10 to Toronto HLA Lab, 10 to Provincial Samples) Lavender (EDTA) Pink (EDTA) or 2 Include on Label: Ensure lot # and expiry date are visible - TGLN # - DOB - Date of draw - Time of draw - OTDC Initials (if space) Send to: Toronto HLA Lab: 200 Elizabeth Street 11E-444 Toronto ON M5G 2C4 Provincial Samples: Provincial Resource Centre 483 Bay St 4th Floor SOUTH Toronto ON M5G 2C9 Who delivers? Taxi (to Air Canada Cargo) 807-577-1193 Account TRILLIUM Where does delivery service pick up? Arranged at time of call OTDC may also drop off directly at Air Canada Cargo January 3, 2020

  21. Windsor Serology and HLA Colour # of tubes Yellow (ACD) 10 8 Pink (EDTA) or Lavender (EDTA) + 1 WNV (May October) Red 1 Gold (SST) 3 10 Yellow (ACD) Provincial Samples DO NOT DRAW until instructed by PRC Send to PRC when instructed (address below) Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC initials (if space) Send to: London Health Sciences Centre Core Lab University Hospital 339 Windermere Road London ON N6A 5A5 Provincial Samples: TGLN Provincial Resource Centre 483 Bay St 4th Floor SOUTH Toronto ON M5G 2C9 Who delivers? Apple Courier 1-888-942-7753 Account 1170 Where does delivery service pick up? Arranged at time of call - Driver comes to ICU January 3, 2020

  22. NAT Testing - if ordered after initial Serology Bloods have been sent Colour Volume 2 Lavender EDTA or Pink EDTA (minimum of 2.5 ml) Include on Label: Ensure tube lot # and expiry date are visible - TGLN # - DOB - Diluted/Undiluted - Date of draw - Time of draw - OTDC Initials (if space) Send to: Toronto Medical Laboratories 600 University Ave, Room 1470 (Serology) Toronto ON M5G 1X5 Who delivers? As per regional rules Where does delivery service pick up? As per regional rules Special Considerations: TML must be aware of incoming sample to ensure prompt results OTDC to Notify PRC re: ETA of NAT sample arrival to TML Note: NAT samples are usually run once daily: between 0930-1000 January 3, 2020

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