Precision Fermentation is a specialized bioprocessing technique that utilizes microbes as cell factories to produce specific functional ingredients like proteins, vitamins, enzymes, and more. This innovative technology addresses the challenges of increasing food demands in a sustainable way, offering solutions for plant-based, algae-based, insect-based, and cell-based food production systems. Commercial case studies demonstrate how precision fermentation is revolutionizing the food industry by creating animal-free and sustainable alternatives to traditional products. The process value chain of precision fermentation involves strain engineering, lab-scale and pilot-scale process development, and commercial-scale production, supported by technical and societal considerations.

• Precision Fermentation
• Sustainable Food Production
• Bioprocessing Technology
• Microbial Cell Factories
• Plant-Based Alternatives

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Precision Fermentation in Sustainable Food Production

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1. Everything You Need to Know About Precision Fermentation Joe Liu (Ph.D) CSIRO, Agriculture & Food

2. The Big Drivers ~ 10 b +70% Population by 2050 More Food Needed By 2050 Other Increasing Consumer Demands Unsustainable Ag&Food systems Food Security

3. Precision Fermentation Precision Fermentation A form of specialized bioprocessing that uses microbes (usually GMO) as cell factories for producing specific functional ingredients, capable of producing proteins, vitamins, enzymes, natural food colour, and fats etc.

4. How does PF fit with other novel food production systems o Plant-Based o Algae-Based o Insect-Based o Microbial Biomass Fermentation o Cell-Based (Cultivated Meat) Lipid Heme Food Colour B12 o Precision Fermentation

5. Commercial Case Studies Nourish Ingredients Precision fermentation technology to produce animal fats without using animal products or palm/coconut oil Animal-free fermented fat as flavour and functional ingredients supplied to other next-gen foods such as plant-based, alternative non-dairy milk Cell-cultured meat meets animal-free fermented fat collaboration between cultured meat company Vow and Nourish Ingredients

6. Commercial Case Studies Eden Brew Synbio technology in yeast to produce milk proteins through precision fermentation Precision fermentation processes commercial-scale development Collaboration with local dairy giant Norco. Co-operative Limited to launch animal-free dairy products

7. Commercial Landscape Global

8. The Process Value Chain of Precision Fermentation Strain Engineering Lab-scale Process Development (Flask to ~20L with Lab DSP) Pilot-scale Process Development (100L to 2,000L with Polit DSP) Demo- and Commercial- scale Process Development (10,000L to 500,000L + with DSP) TEA LCA Societal Food Formulation and Processing, Nutrition, Digestion, Sensory etc. Regulatory Science Societal Science (TEA, LCA, Consumers etc.)

9. Challenges & Solutions Strain Engineering CSIRO Food Innovation Centre(Werribee) Precision Fermentation Pilot Plant,400 Liter fermentation with DSP, Food Grade Facility Lab-scale Process Development (Flask to ~20L with Lab DSP) Pilot-scale Process Development (100L to 2,000L with Polit DSP) Demo- and Commercial- scale Process Development (10,000L to 500,000L + with DSP) Queensland University of Technology (QUT), Mackay Renewable Biocommodities Pilot Plant Precision Fermentation, 2,000 L fermentation with DSP, Food Grade Facility Food Formulation and Processing, Nutrition, Digestion, Sensory etc. Regulatory Science Societal Science (TEA, LCA, Consumers etc.) Cauldron Ferm (NSW) CDMO for precision fermentation, 10,000 L to 100,000 s Liters fermentation with DSP.

10. Challenges & Solutions Strain Engineering CSIRO A&F Engagement with FSANZ and relevant regulators Lab-scale Process Development (Flask to ~20L with Lab DSP) Pilot-scale Process Development (100L to 2,000L with Polit DSP) Ag & F waste as fermentation feedstock, Techno-economic analysis, Consumer acceptance GMO technology, Price Parity, Market access etc. Demo- and Commercial- scale Process Development (10,000L to 500,000L + with DSP) Food Formulation and Processing, Nutrition, Digestion, Sensory etc. Advanced Engineering Biology FSP Future Protein Mission Science Impact Regulatory Science Innovative Bioproduction Platforms Data-driven Molecular Design Interdisciplinary Decision-making Plant-based protein Sustainable animal protein Novel protein production system Societal Science (TEA, LCA, Consumers etc.)

11. What next Relationship to other novel food production systems Infrastructure (R&D, Commercial) Science & Technology Whole Value Chain - from genes to plates Food Government & Funding Companies Scale-up infrastructure in Australia Markets / Consumer Demands Putting the pieces together

12. Questions Thank you & Questions