ABE LAB MANUAL
Abe Lab Manual by Doreen Osgood, MS, PhD, serves as a comprehensive guide for agricultural and biological engineering students. This manual covers essential concepts, practical applications, and research methodologies related to the field. Written by the experienced coordinator, it offers valuable insights and instructions to help readers enhance their understanding and skills in the subject. With a focus on hands-on learning and experiential knowledge sharing, this manual is an indispensable resource for both students and professionals in the agricultural and biological engineering domains.
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Presentation Transcript
ABE LAB MANUAL DOREEN OSGOOD, MS,PHD. ABE RI COORDINATOR
CURRENT MANUAL Goals of the ABE curriculum: Engage a diverse population of students Deepen conceptual understandings of science Expand awareness about biotechnology and the nature of scientific research Show the research and development process for biotechnology 2
LAB MANUAL TAB A Amgen biotech Experience & Program introduction Chapter 1: Tools of the trade TAB B Complete sequence Chapter 2: How do you begin to clone a gene? Chapter 3: Building a recombinant plasmid Chapter 4: Making sure you ve got a recombinant plasmid Chapter 5: Getting recombinant plasmids in bacteria TAB C Abridged sequence All above EXCEPT Chapter 3 TAB D Chapter 5B TAB E: Getting what we need 3
LAB MANUAL ABE lab sequence: Complete genetic engineering sequence 3.5 weeks Abridged genetic engineering sequence A 3 weeks Focus on Bacteria sequence B 2 weeks Introduction to biotechnology About 6 days New curriculum: Modular approach can use entire book or just individual chapters 4
STUDENTS Start at Tab A Everyone does About the Amgen Biotech Experience important! (A-1) Please relay to students HOW they are getting to use this kit Then program introduction & tools of the trade (A-3 & A-15) 7
STUDENTS Program introduction (A-3) Very important! Shows real world relevance to a disease process WHY should they care about this curriculum? 8
STUDENTS Each Chapter: Introduction Chapter goals What do you already know? Section purpose Before the lab Materials Methods Lab techniques Stop and think Safety Questions Glossary 9
TEACHER MANUAL Teacher manual is meant to SUPPORT the students Does not have lab procedures restated Appendix: Lab 1.3 optional Clone that gene DNA ladder diagram Bacterial growth predictions 11
LAB MANUAL - OV Curriculum overview BIG IDEA! Connections to academic standards NGSS Assumption of prior knowledge & skills Assessing student learning Lab Safety Materials and Prep 12
LAB MANUAL PROGRAM INTRODUCTION Overview Assumptions of prior knowledge DNA, RNA, proteins Learning goals Assessed outcome Suggested sequence Session 1, 2, etc. Optional lab recommendations DNA extraction Preparation Materials and reading activities Teaching Goes through each of the sessions 13
LAB MANUAL ALL LABS Labs: Overview Assumptions of prior knowledge Learning goals Assessed outcome Suggested sequence Session 1, session 2, etc. Preparation Teaching (based on suggested sessions) Optional Flowcharts Suggestions: Lab notebook Flow charts laminated on tables 14
LAB MANUALS Manuals All sequences available online for print Website; curriculum; teacher/student guides Teacher guide password protected: merlot Do NOT write in the student books If no longer using the program PLEASE return all lab manuals 15
