Acute Eye Irritation Test Procedure for Pharmacology Study
Utilizing 6 rabbits, this practical involves introducing a test substance into one eye while the other acts as a control. Evaluation of ulceration, corneal opacity, iris inflammation, and conjunctival inflammation is conducted at 24, 48, and 72 hours post-introduction. The aim is to assess acute eye irritation effects through this controlled experiment.
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Presentation Transcript
Practical B. Pharm VI Sem Pharmacology III Aim/Object: Acute Eye irritation test
Instructions - 6 Rabbits have to be taken. - A prescribed amount of test substance should be introduced in one (left) eye of all 6 animals, whereas second (right) eye should considered as control. - Following Response is evaluated after 24, 48 and 72 hrs. - Ulceration - - Opacity of Cornea - - Inflammation of Iris - - Inflammation of conjunctiva
