Advanced Brewery Calculations for Yeast Management
Explore detailed calculations for yeast pitching rates, viability assessments, and total yeast cell requirements in brewing processes. Learn how to determine the necessary volume and weight of yeast slurry based on specific wort characteristics and batch sizes. Enhance your brewing techniques with precise yeast management strategies.
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Calculations - Volume _____ Plato wort x 1x106viable cells/mL wort = viable cells/mL wort = 1 Plato _____ bbl wort x 117.35L wort x 1000mL wort x viable cells = total cells needed = ___ 1bbl wort 1L wort mL wort (average # cells counted)(5)(dilution) = yeast cells/mL of slurry = Chamber volume (0.0001mL3) _________ Total cells needed = mL yeast slurry required = Yeast cells/mL of slurry Convert to gallons mL slurry x 1gal slurry = gal slurry = 3785 mL slurry www.zymologylabs.com
Calculations - Weight _____ Plato wort x 1x106 viable cells/g wort = viable cells/mL wort = 1 Plato _____ bbl wort x 117.35L wort x 1000mL wort x viable cells = total cells needed = ___ 1bbl wort 1L wort mL wort (average # cells counted)(5)(dilution) = yeast cells/g of slurry = _________ Chamber volume (0.0001mL3) Total cells needed = g yeast slurry required = Yeast cells/g of slurry Convert to pounds g slurry x 1lb slurry = lbs slurry 453.592 g slurry www.zymologylabs.com
Pitch Rate Example: For a 12 P beer you would need 12,000,000 viable cells/mL wort 12 x 1x106viable cells/mL wort = 12,000,000 viable cells/mL wort 1 Plato Total cells needed: bbls wort x 117.35L wort x 1000mL wort x viable cells = total cells needed 1bbl wort 1L wort mL wort Example: If you are brewing 5 bbls you need 7.041x1012 cells 5bbls wort x 117.35L wort x 1000mL wort x 1.2x107= 7.041x1012cells 1bbl wort 1L wort mL wort www.zymologylabs.com
Yeast cells/mL or g slurry: (cell count)(5)(dilution) = yeast cells/g slurry Chamber volume (0.0001 mL3) Example: if you count 100 cells in the four corner and one center squares on the hemocytometer using a 1:100 dilution of yeast slurry (100cells)(5)(100) = 5x108 yeast cells/g slurry (0.0001mL) www.zymologylabs.com
Total cells needed = amount of yeast slurry required (g) Yeast cells/g slurry Example: 7.041x1012 cells = 1.4082x104 g 5x108 yeast cells/g slurry g slurry x 1lb slurry = lbs slurry 453.592 g slurry Example: 1.4082x104 g slurry x 1lb slurry = 31 lbs slurry 453.592 g slurry www.zymologylabs.com
Viability total # cells - # blue cells x 100 = % viability total # cells Example: If you count 4 dead cells out of 200 200 4 x 100 = 98% viability 200 31lbs yeast slurry x .2 = .6 lbs So you need to pitch 31.6 lbs to account for the dead yeast www.zymologylabs.com
