Advanced Diagnostic Testing in Microbiology and Virology
Offering a wide range of investigations including ELISA, rapid tests, Real-Time Micro PCR, viral load testing, and in-depth analysis of HIV, Hepatitis B, and HCV. Stay informed about the latest advancements in diagnostic technologies and disease monitoring.
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Presentation Transcript
DEPARTMENT OF MICROBIOLOGY VIROLOGY LABORATORY
INVESTIGATIONS DONE ELISA for : Hepatitis B surface Ag (HBsAg) Hepatitis C Virus (HCV) Hepatitis A Virus (HAV) Hepatitis E Virus (HEV) Human Immunodeficiency Virus (HIV) Dengue TORCH (Toxoplasmosis, Rubella, Cytomegalovirus & Herpes) Japanese Encephalitis Chikungunya Anti Hepatitis B surface Ab(Anti HBsAb)
Rapid Test: Dengue NS1 Ag, IgM, IgG HBsAg HCV HIV Chikungunya CD4 count
Introduction of Newer Diagnostic test Real Time Micro PCR a chip based PCR technology for H1N1 testing.
Future prospective: Viral load--Testing for Hepatitis B viral load (HBV-DNA)
HIV INVESTIGATIONS 9000 8412 7829 8000 7000 6000 5514 4889 5000 4588 4107 4087 4000 3319 3292 3000 2544 2513 2000 993 1000 0 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016
HIV INVESTIGATIONS 12000 10000 8000 Total tests 6000 Reactive 4000 2000 0 2015 2016 2017 2018 2019 2020
HEPATITIS B INVESTIGATIONS 20000 18000 367 16000 209 14000 181 12000 210 157 10000 140 16949 8000 14078 12089 6000 11284 10095 9257 4000 2000 0 201011 2012 2013 2014 2015 2016
HEPATITIS B INVESTIGATIONS 20000 18000 16000 14000 12000 Total tests 10000 Reactive 8000 6000 4000 2000 0 2015 2016 2017 2018 2019 2020
HCV INVESTIGATIONS 20000 18000 78 16000 138 14000 82 12000 63 97 10000 35 16949 8000 14078 12089 6000 11284 10095 9257 4000 2000 0 201011 2012 2013 2014 2015 2016
HCV INVESTIGATIONS 20000 18000 16000 14000 12000 Total tests 10000 Reactive 8000 6000 4000 2000 0 2015 2016 2017 2018 2019 2020
DENGUE INVESTIGATIONS 6000 5000 4000 Total tests 3000 Positive 2000 1000 0 2015 2016 2017 2018 2019 2020
EQUIPMENTS ELISA Reader Dispenser & Washer. ELISA washer Deep freezer Pima CD4 counter Refrigerator Incubator Autopipettes True Lab Uno Dx Real Time Quantitative micro PCR Analyzer
PROJECTS Completed Spectrum of blood borne viral hepatitis with special reference to HCV genotyping.
PROJECTS Completed Research Project: Seroprevalence of Antibodies to Hepatitis C Virus in a Hospital Based Population A Study from Western Maharashtra Seroprevalence of Hepatitis B Antigen in a Teaching Hospital of Western Maharashtra. Trend of Seroprevalence of HIV, HBsAg & HIV Antibodies among Pregnant Mothers Attending a Rural Teaching Hospital. Risk of Co-Existence of HIV, HBV and HCV Infection among Pregnant Women From Karad. Socio-demographic Profile of Seropositive HBsAg Patients Attending Tertiary Care Hospital of Rural Maharashtra.
PUBLICATIONS Patil SR, Ghorpade MV, Patil SS, Pawar SK, Mohite ST. Seroprevalence of Hepatitis-B surface antigen among the patients reporting at tertiary care Hospital from India. Bangladesh Journal of Medical Science. 2016 Nov 3;15(3):455-9. Patil SR, Patil SS, Shinde RV, Karande GS, Patil HV, Mane PM. Seropositivity and Risk Factors of Hepatitis B in Maharashtra, India. JOURNAL OF KRISHNA INSTITUTE OF MEDICAL SCIENCES UNIVERSITY. 2016 Jan 1;5(1):82-90. Patil SR, Ghorpade M V , Patil SS, Shinde R V., Mohite S T. Seroprevalence of Antibodies to the Hepatitis C virus in a Hospital Based Population: A study from western Maharashtra, India . International Journal of Collaborative Research on Internal Medicine & Public Health 2014 ; 6 (4):102-108 Patil SS, Patil SR, Durgawale PM, Patil AG. A study of the outbreak of Chikungunya fever. Journal of clinical and diagnostic research: JCDR. 2013 Jun;7(6):1059.
VIROLOGY LABORATORY ELISA : Hepatitis B surface Ag (HBsAg) Hepatitis C Virus (HCV) Hepatitis A Virus (HAV) Hepatitis E Virus (HEV) Anti Hepatitis B surface Ab(Anti HBsAb) Human Immunodeficiency Virus (HIV) Dengue TORCH Chikungunya Japanese Encephalitis Immunochromatography : Dengue, HBsAg, HCV, HIV, Chikungunya. CD4 count
Virology laboratory (contd) Real time PCR future prospectives. - Viral load - Viral genotyping
KIMS DIAGNOSTICS MICROBIOLOGY SECTION Parameters accreditated by NABL HBsAg ELISA for Antigen detection HCV ELISA - for Antibody detection HIV ELISA - for Antibody detection Rapid test for HIV a) Tridot b) Retroquic External Quality Assessment Scheme (EQAS) by Virology EQAS, Christian Medical college, Vellore, Tamilnadu
THANK YOU
