Antibiotic Sensitivity in Nosocomial Infections: Understanding Microorganism Resistance
Nosocomial infections pose a significant risk in healthcare settings, with ESBL-producing bacteria being a major concern. The identification of causative bacteria and their antibiotic resistance profile is crucial for effective treatment.
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Antibiotics sensitivity of microorganism causing nosocomial infections 3rdInternational Conference on Clinical Microbiology & Microbial Genomics Tea Koiava Batumi Shota Rustaveli State University, Georgia
Internal infections The most frequent reason for a mortality of hospital patients is internal infections which are associated with general medical manipulations; 2 million people become ill from this infection and 5-10 % of them die annually.
Nosocomial infections .are a major problem today, not only in developing countries but also in the major developed countries; Antibiotic use is one of the important factor in the development of nosocomial infections;
microorganisms causing nosocomial infection in most cases belong to the ESBL producing bacteria and are resistant to several antibiotics
ESBL bacteria vary according to regions and countries Pseudomonas aeruginosa Klebsiella E. coli Ascinetobacter are mostly found among the agents of the infection.
The identification of bacterial spectrum of agents of these infections is incompletely carried out and the antibiotic therapy has been determined spontaneously the etiologic
. The goal of our research was identify the causative bacteria of nosocomial infection in several hospitals in Adjara to study the profile of antibiotic resistance.
Materials Sputum Urine Blood Biological Fluids
Methods Culturing of a culture into culture medium; Obtaining of pure culture; Identification of cultures by using the API tests; Antibiotic resistance was determined by Bauer-Kirby Disk Diffusion method.
Resultes 100 samples were taken the first 48 hours and totally examined. 81 from that for bacteria were positive
Most numerous among the agents of nosocomial infection were Acinetobacter E. coli Pseudomonas Aeruginosa Klebsiella Pneumonia
Urine Sterile Kl. Pneumoniae Ps. Aeruginosa 11% 45% 44%
Biological fluids E.coli Acinetobacter Ps. Aeruginosa 24% 41% 35%
Suptum E.coli Acinetobacter Ps. Aeruginosa Kl. Pneumoniae Kl. Oxytoca 11% 11% 26% 30% 22%
Antibiotic Resistance Profile Cefalosporine Ceftriaxone, Cefepime, Piperacillin, Aztreonam, Augmentin, Penicillin, Oxacillin Ciprofloxacin, Colistin, Imipenem, Meropenem, Gentamicin, Amikacin, Ampicillin / sulbactam, Co-trimoxazole, Kloramfenikol, Fosfomycin
Antibiotic Resistance Profile 60 50 40 30 20 10 0 Ceftriaxone Cefepime Piperacilin Aztreonam Imipenem Meropenem R S M
Antibiotic Resistance Profile 90 80 70 60 50 40 30 20 10 0 Augmentin Penicilin Ampicilin Biol. Fluids Sputum Urine
All isolantes of the gram- negative bacteria were resistant to Augmentin Ampicilin Penicilin
Conclusions Augmentin, Ampicilin, Penicilin are antibiotics usingmassively in Georgia; Supposedly use of these drug as, illegal in most cases without a prescription (these drugs are freely sold in pharmacies) contributes to the formation of antibiotic resistance.
In the future We are playing detail genetic screening for: bla SHV bla CTX-M bla TEM
Thank You for Your attention!