Comprehensive Analysis of MS Qual/Quant QC Mix for Platform Characteristics Assessment

The MS Qual/Quant QC Mix (Sigma Aldrich) is designed for daily use in evaluating platform characteristics such as repeatability, system stability, and inter-/intra-platform comparisons. The content includes XIC analysis for peptides, a detailed table showing the difference in peak ratios, and correlation curves comparing theoretical ratios with standard ratios. These analytical tools provide valuable insights for improving analytical processes and ensuring data accuracy in mass spectrometry.

• MS Qual/Quant QC Mix
• Sigma Aldrich
• Platform Characteristics
• Peptide Analysis
• Correlation Curves

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Uploaded on Aug 04, 2024 | 1 Views

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1. MSQC1, MS Qual/Quant QC Mix

2. MS Qual/Quant QC Mix (Sigma- Aldrich) is intended for daily use to assess platform characteristics, including: Repeatability System stability (drift, chromatography, signal intensity, sensitivity, etc.) Inter- and intra-platform and lab comparisons

3. XIC for 3 peptides with their havey versions (MSQC1 standard QC from sigma)

4. XIC for 3 peptides with their havey versions (MSQC1 standard QC from sigma)

5. XIC for 5 peptides with their havey versions (MSQC1 standard QC from sigma)

6. Table 2. shows the difference between RatioToStandard Vs Theoretical ratios. (Results Report, from Skyline) Peptide Sequence PeptidePeakFoundRatio RatioToStandard (L/H) Theoretical ratio 2 VLDALQAIK 1 2.0789 1 GGPFSDSYR 1 0.9873 50 SADFTNFDPR 0.67 34.6957 10 AVQQPDGLAVLGIFLK 0.67 5.2727 1 ALIVLAHSER 0.67 0.7895 2 EGHLSPDIVAEQK 1 1.2599 50 GYSIFSYATK 1 29.5 10 ESDTSYVSLK 0.83 7.3286 0.5 FEDENFILK 1 0.2171 1 VSFELFADK 0.83 0.4646 50 NLSVEDAAR 0.67 13.6667 10 FSTVAGESGSADTVR 0.5 2.5

7. Correlations curve between theoretical Vs Ratio toStandard Run, with R=0.8179 70 60 R = 0.8179 50 40 30 20 10 0 0 10 20 30 40 50 60 70 Theoretical Ratio

8. Table 2. shows the difference between RatioToStandard Vs Theoretical ratios. (Results Report, from Skyline) Peptide Sequence PeptidePeakFoundRatio RatioToStandard (L/H) Theoretical ratio 2 VLDALQAIK 1 2.0789 1 GGPFSDSYR 1 0.9873 50 SADFTNFDPR 0.67 34.6957 10 AVQQPDGLAVLGIFLK 0.67 5.2727 1 ALIVLAHSER 0.67 0.7895 2 EGHLSPDIVAEQK 1 1.2599 50 GYSIFSYATK 1 29.5 10 ESDTSYVSLK 0.83 7.3286 0.5 FEDENFILK 1 0.2171 1 VSFELFADK 0.83 0.4646 10 FSTVAGESGSADTVR 0.5 2.5

9. Correlations curve between theoretical Vs Ratio toStandard Run, with R=0.9796 60 R = 0.9796 50 Ratio toStandard Run 40 30 20 10 0 0 5 10 15 20 25 30 35 40 Theoretical Ratio

10. Sensitivity comparison between 4000 Vs 5500 Qtrap for NLSVEDAAR peptide. Peak area is 5 times higher in 5500 QTRAP 5500 4000

11. Sensitivity comparison between 4000 Vs 5500 Qtrap for NLSVEDAAR peptide. Peak area is 5 times higher in 5500 QTRAP 5500 4000