CRISPR and Nanopore Sequencing Results Analysis

CRISPR and Nanopore Sequencing Results Analysis
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In this study, Naisha Shah from America and Fanny Alory from France, mentored by Augustas Stanaitis from Lithuania, conducted CRISPR/Cas9 experiments followed by Nanopore sequencing. The results revealed successful gRNA synthesis in Step One and DNA cleavage in Step Two. However, Step Three showed challenges in DNA library preparation and sequencing. The analysis highlights important observations and insights gained from the experimental process.

  • CRISPR
  • Nanopore Sequencing
  • gRNA Synthesis
  • DNA Cleavage
  • DNA Library
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Uploaded on Feb 18, 2025 | 0 Views

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  1. TRAVEL CODE QUIZ QUIZ QUIZ

  2. LEVEL 1 QUIZ Question 1: Question 1: When you re on the bus, what should you do? When you re on the bus, what should you do? - Change seats to sit next to your friends? Change seats to sit next to your friends? - Play in the aisle? Play in the aisle? - Stay in your seat for your whole journey? Stay in your seat for your whole journey? NEXT QUESTION NEXT QUESTION

  3. LEVEL 1 QUIZ Question 1: Question 1: When you re on the bus, what should you do? When you re on the bus, what should you do? - Change seats to sit next to your friends? Change seats to sit next to your friends? - Play in the aisle? Play in the aisle? - Stay in your seat for your whole journey? Stay in your seat for your whole journey? Unlucky, wrong answer, try again. Unlucky, wrong answer, try again. BACK Hint: Stay in seat. Hint: Stay in seat. BACK NEXT QUESTION NEXT QUESTION

  4. LEVEL 1 QUIZ Question 1: Question 1: When you re on the bus, what should you do? When you re on the bus, what should you do? - Change seats to sit next to your friends? Change seats to sit next to your friends? - Play in the aisle? Play in the aisle? - Stay in your seat for your whole journey? Stay in your seat for your whole journey? Well done Well done you know your travel code! you know your travel code! BACK BACK NEXT QUESTION NEXT QUESTION

  5. LEVEL 1 QUIZ Question 2: Question 2: If you are getting off the bus and want to cross the road should you If you are getting off the bus and want to cross the road should you - Cross the road close behind the bus? Cross the road close behind the bus? - Cross the road in front of the bus? Cross the road in front of the bus? - Wait until the bus has gone? Wait until the bus has gone? NEXT QUESTION NEXT QUESTION

  6. LEVEL 1 QUIZ Question 2: Question 2: If you are getting off the bus and want to cross the road should you If you are getting off the bus and want to cross the road should you - Cross the road close behind the bus? Cross the road close behind the bus? - Cross the road in front of the bus? Cross the road in front of the bus? - Wait until the bus has gone? Wait until the bus has gone? Unlucky, wrong answer, try again. Unlucky, wrong answer, try again. Hint: Wait Hint: Wait BACK BACK NEXT QUESTION NEXT QUESTION

  7. LEVEL 1 QUIZ Question 2: Question 2: If you are getting off the bus and want to cross the road should you If you are getting off the bus and want to cross the road should you - Cross the road close behind the bus? Cross the road close behind the bus? - Cross the road in front of the bus? Cross the road in front of the bus? - Wait until the bus has gone? Wait until the bus has gone? Well done you know your travel code! Well done you know your travel code! BACK BACK NEXT QUESTION NEXT QUESTION

  8. LEVEL 1 QUIZ Question 3: Question 3: If there s a seat belt, what should you do? If there s a seat belt, what should you do? - Wear it? Wear it? - Ignore it? Ignore it? - Play with it? Play with it? NEXT QUESTION NEXT QUESTION

  9. LEVEL 1 QUIZ Question 3: Question 3: If there s a seat belt, what should you do? If there s a seat belt, what should you do? - Wear it? Wear it? - Ignore it? Ignore it? - Play with it? Play with it? BACK Unlucky, wrong answer, try again. Unlucky, wrong answer, try again. Hint: Hint: You would do the same in a car You would do the same in a car . BACK . NEXT QUESTION NEXT QUESTION

  10. LEVEL 1 QUIZ Question 3: Question 3: If there s a seat belt, what should you do? If there s a seat belt, what should you do? - Wear it? Wear it? - Ignore it? Ignore it? - Play with it? Play with it? BACK Well done Well done you re very good! you re very good! BACK NEXT QUESTION NEXT QUESTION

  11. LEVEL 1 QUIZ Question 4: Question 4: If the bus is moving, when should you talk to the driver? If the bus is moving, when should you talk to the driver? - Only in an emergency? Only in an emergency? - To ask him to drop you off early? To ask him to drop you off early? - To tell him a joke? To tell him a joke? NEXT QUESTION NEXT QUESTION

  12. LEVEL 1 QUIZ Question 4: Question 4: If the bus is moving, when should you talk to the driver? If the bus is moving, when should you talk to the driver? - Only in an emergency? Only in an emergency? - To ask him to drop you off early? To ask him to drop you off early? - To tell him a joke? To tell him a joke? Well done! You are brilliant Well done! You are brilliant BACK BACK NEXT QUESTION NEXT QUESTION

  13. LEVEL 1 QUIZ Question 4: Question 4: If the bus is moving, when should you talk to the driver? If the bus is moving, when should you talk to the driver? - Only in an emergency? Only in an emergency? - To ask him to drop you off early? To ask him to drop you off early? - To tell him a joke? To tell him a joke? Unlucky try again. Unlucky try again. BACK Hint:: Only when Hint:: Only when BACK NEXT QUESTION NEXT QUESTION

  14. LEVEL 2 QUIZ Question 1: Question 1: What s the best way for you and your mate to cycle home? What s the best way for you and your mate to cycle home? - Side by side? Side by side? - In between the buses? In between the buses? - Tight into the kerb on the left side of the road? Tight into the kerb on the left side of the road? NEXT QUESTION NEXT QUESTION

  15. LEVEL 2 QUIZ Question 1: Question 1: What s the best way for you and your mate to cycle home? What s the best way for you and your mate to cycle home? - Side by side? Side by side? - In between the buses? In between the buses? - Tight into the kerb on the left side of the road? Tight into the kerb on the left side of the road? Well done that is correct! Well done that is correct! BACK BACK NEXT QUESTION NEXT QUESTION

  16. LEVEL 2 QUIZ Question 1: Question 1: What s the best way for you and your mate to cycle home? What s the best way for you and your mate to cycle home? - Side by side? Side by side? - In between the buses? In between the buses? - Tight into the kerb on the left side of the road? Tight into the kerb on the left side of the road? Unlucky, wrong answer. Try again! Unlucky, wrong answer. Try again! BACK Hint:: close to Hint:: close to BACK NEXT QUESTION NEXT QUESTION

  17. LEVEL 2 QUIZ Question 2: Question 2: If you ve stopped your bike at a set of traffic lights, when should you move forward? If you ve stopped your bike at a set of traffic lights, when should you move forward? - As soon as the lights change to amber? As soon as the lights change to amber? - Go as soon as you see a green light? Go as soon as you see a green light? - Start moving forward when the lights are red? Start moving forward when the lights are red? NEXT QUESTION NEXT QUESTION

  18. LEVEL 2 QUIZ Question 2: Question 2: If you ve stopped your bike at a set of traffic lights, when should you move forward? If you ve stopped your bike at a set of traffic lights, when should you move forward? - As soon as the lights change to amber? As soon as the lights change to amber? - Go as soon as you see a green light? Go as soon as you see a green light? - Start moving forward when the lights are red? Start moving forward when the lights are red? Incorrect Incorrect try again try again Hint:: The hulk Hint:: The hulk BACK BACK NEXT QUESTION NEXT QUESTION

  19. LEVEL 2 QUIZ Question 2: Question 2: If you ve stopped your bike at a set of traffic lights, when should you move forward? If you ve stopped your bike at a set of traffic lights, when should you move forward? - As soon as the lights change to amber? As soon as the lights change to amber? - Go as soon as you see a green light? Go as soon as you see a green light? - Start moving forward when the lights are red? Start moving forward when the lights are red? Correct! You re getting good at this Correct! You re getting good at this BACK BACK NEXT QUESTION NEXT QUESTION

  20. LEVEL 2 QUIZ Question 3: Question 3: What should you do if you see any poor behaviour or bullying? What should you do if you see any poor behaviour or bullying? - Ignore it? Ignore it? - Join in and do the same? Join in and do the same? - Tell a teacher, a parent or driver? Tell a teacher, a parent or driver? NEXT QUESTION NEXT QUESTION

  21. LEVEL 2 QUIZ Question 3: Question 3: What should you do if you see any poor behaviour or bullying? What should you do if you see any poor behaviour or bullying? - Ignore it? Ignore it? - Join in and do the same? Join in and do the same? - Tell a teacher, a parent or driver? Tell a teacher, a parent or driver? Correct again! Well done Correct again! Well done BACK BACK NEXT QUESTION NEXT QUESTION

  22. LEVEL 2 QUIZ Question 3: Question 3: What should you do if you see any poor behaviour or bullying? What should you do if you see any poor behaviour or bullying? - Ignore it? Ignore it? - Join in and do the same? Join in and do the same? - Tell a teacher, a parent or driver? Tell a teacher, a parent or driver? Oops Oops wrong answer. Try again wrong answer. Try again BACK Hint : You need to tell an adult Hint : You need to tell an adult BACK NEXT QUESTION NEXT QUESTION

  23. LEVEL 2 QUIZ Question 4: Question 4: If you re rushing to school and the pavement is busy, do you: If you re rushing to school and the pavement is busy, do you: - Walk in the road? Walk in the road? - Walk in the cycle lane? Walk in the cycle lane? - Stick to the pavement? Stick to the pavement? NEXT QUESTION NEXT QUESTION

  24. LEVEL 2 QUIZ Question 4: Question 4: If you re rushing to school and the pavement is busy, do you: If you re rushing to school and the pavement is busy, do you: - Walk in the road? Walk in the road? - Walk in the cycle lane? Walk in the cycle lane? - Stick to the pavement? Stick to the pavement? Good guess but have another go Good guess but have another go BACK Hint : This is to keep you safe Hint : This is to keep you safe BACK NEXT QUESTION NEXT QUESTION

  25. LEVEL 2 QUIZ Question 4: Question 4: If you re rushing to school and the pavement is busy, do you: If you re rushing to school and the pavement is busy, do you: - Walk in the road? Walk in the road? - Walk in the cycle lane? Walk in the cycle lane? - Stick to the pavement? Stick to the pavement? Correct! Great job! Correct! Great job! BACK BACK NEXT QUESTION NEXT QUESTION

  26. LEVEL 3 QUIZ Question 1: Question 1: Where s the best place to throw away sweet wrappers? Where s the best place to throw away sweet wrappers? - Down the drain in the gutter? Down the drain in the gutter? - In In a bin? a bin? - Over a hedge? Over a hedge? NEXT QUESTION NEXT QUESTION

  27. LEVEL 3 QUIZ Question 1: Question 1: Where s the best place to throw away sweet wrappers? Where s the best place to throw away sweet wrappers? - Down the drain in the gutter? Down the drain in the gutter? - In In a bin? a bin? - Over a hedge? Over a hedge? Correct! Great job Correct! Great job BACK BACK NEXT QUESTION NEXT QUESTION

  28. LEVEL 3 QUIZ Question 1: Question 1: Where s the best place to throw away sweet wrappers? Where s the best place to throw away sweet wrappers? - Down the drain in the gutter? Down the drain in the gutter? - In In a bin? a bin? - Over a hedge? Over a hedge? Not quite try again! Not quite try again! BACK Hint : You have these in your house Hint : You have these in your house BACK NEXT QUESTION NEXT QUESTION

  29. LEVEL 3 QUIZ Question 2: Question 2: What s the best way to cross the road? What s the best way to cross the road? - Walk until you get to a proper crossing? Walk until you get to a proper crossing? - Zig Zig zag zag through the cars? through the cars? - Stroll out with your mates and hope the traffic stops? Stroll out with your mates and hope the traffic stops? NEXT QUESTION NEXT QUESTION

  30. LEVEL 3 QUIZ Question 2: Question 2: What s the best way to cross the road? What s the best way to cross the road? - Walk until you get to a proper crossing? Walk until you get to a proper crossing? - Zig Zig zag zag through the cars? through the cars? - Stroll out with your mates and hope the traffic stops? Stroll out with your mates and hope the traffic stops? Correct! You re becoming an expert Correct! You re becoming an expert BACK BACK NEXT QUESTION NEXT QUESTION

  31. LEVEL 3 QUIZ Question 2: Question 2: What s the best way to cross the road? What s the best way to cross the road? - Walk until you get to a proper crossing? Walk until you get to a proper crossing? - Zig Zig zag zag through the cars? through the cars? - Stroll out with your mates and hope the traffic stops? Stroll out with your mates and hope the traffic stops? Almost try again Almost try again BACK BACK Hint : Walking Hint : Walking NEXT QUESTION NEXT QUESTION

  32. LEVEL 3 QUIZ Question 3: Question 3: Who is the Travel Code for? Who is the Travel Code for? - Just for bus users? Just for bus users? - Just those that take the bus or walk? Just those that take the bus or walk? - All learners? All learners? NEXT QUESTION NEXT QUESTION

  33. LEVEL 3 QUIZ Question 3: Question 3: Who is the Travel Code for? Who is the Travel Code for? - Just for bus users? Just for bus users? - Just those that take the bus or walk? Just those that take the bus or walk? - All learners? All learners? Correct! It is for everyone to use. Correct! It is for everyone to use. BACK BACK NEXT QUESTION NEXT QUESTION

  34. LEVEL 3 QUIZ Question 3: Question 3: Who is the Travel Code for? Who is the Travel Code for? - Just for bus users? Just for bus users? - Just those that take the bus or walk? Just those that take the bus or walk? - All learners? All learners? Good guess, try again. Good guess, try again. BACK Hint : You and your friends Hint : You and your friends BACK NEXT QUESTION NEXT QUESTION

  35. LEVEL 3 QUIZ Question 4: Question 4: If someone is in your way on a zebra crossing, do you: If someone is in your way on a zebra crossing, do you: - Push past? Push past? - Politely wait for them to cross first? Politely wait for them to cross first? - Cross on the road instead? Cross on the road instead? FINISH FINISH

  36. LEVEL 3 QUIZ Question 4: Question 4: If someone is in your way on a zebra crossing, do you: If someone is in your way on a zebra crossing, do you: - Push past? Push past? - Politely wait for them to cross first? Politely wait for them to cross first? - Cross on the road instead? Cross on the road instead? Not quite try again Not quite try again BACK Hint : You need to respect other people Hint : You need to respect other people BACK FINISH FINISH

  37. LEVEL 3 QUIZ Question 4: Question 4: If someone is in your way on a zebra crossing, do you: If someone is in your way on a zebra crossing, do you: - Push past? Push past? - Politely wait for them to cross first? Politely wait for them to cross first? - Cross on the road instead? Cross on the road instead? Correct! Great effort. Correct! Great effort. BACK BACK FINISH FINISH

  38. CONGRATULATIONS You have completed the Travel Code Quiz. You have completed the Travel Code Quiz. CLICK HERE TO GO BACK TO THE START CLICK HERE TO GO BACK TO THE START

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