CRISPR Results and Detailed Analysis
Explore the detailed process and analysis of CRISPR results, from gRNA production to sequencing overview and implicate bases percentage. Discover the close-up of the sequence and the conclusion drawn from the experiment.
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Presentation Transcript
Group 14 Lucia Bizo ov (SK) Aris Kapetanakis (GR) Max Paul (DK)
Results Results of CRISPR/ of CRISPR/Cas Cas step 1 step 1 gRNA St0, submerged in ice In wells 7 and 8 we have our samples. In well 8 you can observe a successful production of gRNA. However, in well 7 there is nothing to observe as there was no gRNA produced.
Results Results of CRISPR/ of CRISPR/Cas Cas step 2 step 2 In wells 7 and 8 you can see our samples. In well 8 we linearized the plasmid, meaning we cut it with the Cas9 protein at a precise location with the help of gRNA, which resulted in it migrating faster. In well 7, however, we have a non-cut plasmid which did not travel as far in the same time frame since it was not cut. Linearised plasmid Non-cut plasmid
Sequencing overview What we observed: Coloured columns White zones
Detailed Analysis 11 15 16 17 1819202122 23 24 3 4 5 6 7 8 9 12 13 14 1 2 25 10
Detailed Analysis 29 26 28 32 33 34 31 27 30
Detailed Analysis 39 40 36 37 38 35
Detailed Analysis 39 40 36 37 38 35 40 imprecise bases out of 4361!!! (0,92%). Extremely precise!!
Close up of sequence Target DNA PAM sequence
Group 14 Lucia Bizo ov (SK) Aris Kapetanakis (GR) Max Paul (DK)
