Development and Validation of HPLC Method for Ursolic Acid in Plasma
A simple and effective HPLC method was developed and validated for the estimation of ursolic acid in drug-free plasma. The method demonstrated specificity, precision, and accuracy over a concentration range of 25.0-150.0 ng/mL. It was found to be accurate, reproducible, and suitable for bioanalytical studies of ursolic acid.
Download Presentation
Please find below an Image/Link to download the presentation.
The content on the website is provided AS IS for your information and personal use only. It may not be sold, licensed, or shared on other websites without obtaining consent from the author. If you encounter any issues during the download, it is possible that the publisher has removed the file from their server.
You are allowed to download the files provided on this website for personal or commercial use, subject to the condition that they are used lawfully. All files are the property of their respective owners.
The content on the website is provided AS IS for your information and personal use only. It may not be sold, licensed, or shared on other websites without obtaining consent from the author.
E N D
Presentation Transcript
Simple and Effective HPLC Method Development and its Validation for Ursolic acid in Drug Free plasma: Application to bioanalytical studies Selvadurai Muralidharan, Jayaraja kumar, Venugopal Vijayan
Objective: chromatographic (HPLC) method was developed for estimation of ursolic acid in drug free human drug free blank plasma. Material and Methods: The current method was used protein precipitating extraction of ursolic acid from blank plasma. Separation was achieved on reversed-phase C18 column (250 4.6 mm, 5 ) and the detection was monitored by UV detector at 220 nm. The optimized mobile phase was used acetonitrile: 0.5% triethyl amine (pH 4.0), in the ratio of 70:30 % v/v at a flow rate of 1.0 mL/min. This linearity was achieved in this method range of 25.0 150.0 ng/ml with regression coefficient range is 0.99. Simple and effective high performance liquid
Results: The present method is suitable in terms of precise, accurate and specific during the study. The simplicity of the method allows for application in laboratories that instruments such as GC MS/MS or LC MS/MS that are costly, time consuming and complicated rather than a simple HPLC UV method. The present method was successfully applied for bioanalytical studies. lack sophisticated analytical
KEYWORDS Bioanalytical studies, HPLC, Ursolic acid, Method Development and Validation.
A simple and sensitive method for the determination of ursolic acid in plasma by HPLC was developed and validated. Adequate specificity, precision and accuracy of the proposed method were demonstrated over the concentration range of 25.0-150.0 ng/mL. The method was accurate, reproducible, specific and applicable to the evaluation of pharmacokinetic profiles of ursolic acid and suitable for the bioanalytical study of Ursolic acid.
