Enzyme Activity Demonstration: Peroxidase Assay Explained
Explore the enzyme activity of peroxidase in various organisms, understand the difference between peroxide and catalase, and learn how to calculate enzyme activity using the extinction coefficient. Discover the importance of peroxidase in breaking down hydrogen peroxide and watch peroxidase assay demonstrations for a comprehensive understanding.
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Presentation Transcript
Experiment 1 To Demonstrate the enzyme activity of peroxidase in given sample
Peroxidase is an enzyme found in a wide variety of organisms, from plants to humans to bacteria. Its function is to break down hydrogen peroxide (H2O2), which is one of the toxins produced as a byproduct of using oxygen for respiration.
Difference between Peroxide & Catalase Peroxidase detoxifies hydrogen peroxide, catalase detoxifies hydroxyl radicals. Catalase produces oxygen, peroxidase does not. Co-factors of peroxidase: heme and Histidine
https://study.com/academy/lesson/lab-13-enzyme-activity.html
Peroxidase Assay https://www.youtube.com/watch?v=ezayzkqZtgk https://www.youtube.com/watch?v=JBClWls3SUk
Calculation The enzyme activity can be calculated according to the following equation: Enzyme activity (Units/L) = ( Abs Total assay volume) / ( t x x l x Enzyme sample volume). Abs= Absorbance Abs = Absorbance of sample Absorbance of Blank t = Time = extinction coefficient of substrates (concentration of substance dissolved in a given solute and measured at a given wavelength) l = the cuvette diameter (1cm)
Extinction coefficient refers to several different measures of the absorption of light in a medium: Attenuation coefficient, sometimes called "extinction coefficient" how strongly a substance absorbs light at a given wavelength, per mass density. The extinction coefficient allows you to determine the remaining amount of substrate after reaching equilibrium.
