Estimation of Hemoglobin Percentage in Human Blood: Practical Method
Discover the role of hemoglobin in blood, learn the principle behind its function, and understand how to estimate hemoglobin percentage using a simple procedure involving N/10 HCl and a haemoglobinometer.
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Examination Percentage in Human Blood of Hemoglobin Class- B.Sc. III Year Practical Time: 03:00-05:00PM Presented by Ashok Kumar Department of Zoology UCOS,MLSU, Udaipur.
Hemoglobin J.F. Engelhart (1825) discovered the hemoglobin in several species. Gilbert confirmed Engelhart in 1925. The oxygen-carrying property of hemoglobin was discovered by H nefeld in 1840. In 1959, Max Perutz and John Kendrew determined the molecular structure of hemoglobin by X-ray crystallography. The role of hemoglobin in the blood was elucidated by French physiologist Claude Bernard.
Principle:- Hemoglobin is a coloured pigment, found in the blood and provid red colour to the blood. It play an important role in the transportation of gases. When hemoglobin is treated with acid, it makes hamatin acid of brown or light pink coloured.
Estimation of Hemoglobin Percentage Requirements- Haemoglobinometer and N/10 HCl, Blood Sample, Cotton, Spirit, Lancet or Needle Procedure- 1. Sterilize middle finger tip with cotton soaked spirit 2. Prick the sterilized finger with a sterilized lancet or needle and discard the 1st drop of blood. 3. Suck the blood up to 0.2 ml mark in pipette. 4. Transfer the blood in tube containing small amount of N/10 HCl (experimental tube). 5. Now add the N/10 HCl drop by drop by comparing the color with adjacent colored tube of instrument (standard tubes).
Observation- By continuing addition of N/10 HCl drop by drop we find the end point on which colour of both the coloured instrument tube is similar to experimental tube and now we evaluate % of Hb in given blood sample.
