Evaluation of Anisakis Presence in Fish Products of Sicily
This study conducted at the National Conference on Public Veterinary Health in Sicily aimed to evaluate the prevalence of Anisakis nematodes in fish products, assess the impact of storage methods on viability, allergy potential, and heavy metal levels, while identifying Anisakis species present in the Mediterranean Sea. The research highlighted the need for improved surveillance systems, establishment of an official database for food-borne hazards, and the potential of Anisakis as a bioindicator for environmental contaminants.
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4 Convegno Nazionale sulla Ricerca in Sanit Pubblica Veterinaria Evaluation of Anisakis presence in fish products of Sicily and survey on the efficiency of storage methods on the viability and allergy potential Antonella COSTA , Antonio VELLA , Andrea MACALUSO , Gaetano CAMMILLERI , Stefania GRACI , Maria Drussilla BUSCEMI , Giovanni CASSATA , Aldo MIGLIAZZO , Alessandro GIUFFRIDA , Vincenzo FERRANTELLI Istituto Zooprofilattico Sperimentale della Sicilia A. Mirri Palermo; Universit degli Studi di Messina Una sanit pubblica veterinaria senza frontiere per affrontare le sfide emergenti Roma, 6 aprile 2017
4 Convegno Nazionale sulla Ricerca in Sanit Pubblica Veterinaria Aims: Evaluate the prevalence, geographical distribution and seasonal occurrence of Anisakidae nematodes in fish products marketed in Sicily; Evaluate differences in mercury accumulation between parasitized and not parasitized fishes; Increase the scientific knowledge about pathology connected with the presence of Anisakis in food production; Materials and Methods: Visual inspection and digestion of the fish samples; Morphological characterization of the larvae; Molecular analysis: PCR-RFLP, Multiplex PCR and Sequencing; Real time PCR method development for processed products; Detection of mercury levels. Una sanit pubblica veterinaria senza frontiere per affrontare le sfide emergenti | Roma, 6 aprile 2017
4 Convegno Nazionale sulla Ricerca in Sanit Pubblica Veterinaria Main results The presence of Anisakidae larve was detected in 463 fish samples (32,6%), while Anisakidae DNA was found only in 3 trasformed fish products (13%). Molecular analysis confirm Anisakis pegreffii as the prevalent species of Mediterranean Sea. Furthermore, the presence of A. simplex s.s., A. physeteris, A. pegreffii/A. simplex s.s. hybrid forms and Hysterothylacium fabri was verified with lower prevalence values. The Real time PCR method has been validated and revealed high sensitivity, specificity, for the determination of Anisakidae DNA in processed fish products. A significant correlation was found between Anisakidae infestation and presence of heavy metals (Hg) levels (p<0.05). Una sanit pubblica veterinaria senza frontiere per affrontare le sfide emergenti | Roma, 6 aprile 2017
4 Convegno Nazionale sulla Ricerca in Sanit Pubblica Veterinaria GAP and future perspectives 1. Establish an official database aimed to identify food-borne hazards to human health; 2. Evaluation of environmental contaminants through the use of Anisakis as bioindicator environmental of heavy metals pollution; 3. Surveillance systems should be established or improved at the level of healthcare institutions both regional and national level. Una sanit pubblica veterinaria senza frontiere per affrontare le sfide emergenti | Roma, 6 aprile 2017
