Examination of Human Blood Group and Rh Factor - Class B.Sc. III Year Practical

This practical session explores the identification of human blood groups and Rh factor through tests based on antigen-antibody reactions. The session covers the types of blood groups according to Karl Landsteiner's discovery and the procedure for testing blood samples using cavity slides and specific antisera. Observations are made based on clumping reactions to determine the blood group. Images illustrate the process and results obtained during the examination.

• Blood Group
• Rh Factor
• Karl Landsteiner
• Antigen
• Antibody

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1. Examination of Human Blood Group and Rh Factor Class- B.Sc. III Year Practical Date: 19.09.2020 Time: 03:00-05:00PM Presented by Manoj Joshi Department of Zoology UCoS,MLSU, Udaipur.

2. Type of Blood Groups Karl Landstenier (1900) discovered Blood group . According to him absence and presence of A and B agglutiongenes or A and B antigens in RBC Blood groups can be classified. S. No. Blood group Antigen Antibody 1 A B A 2 B A B 3 AB A and B - 4 O - a&b

3. Type of Blood Groups Requirements- Cavity slides, Blood sample vials or small glass tubes, Antiserum A, Antiserum B and Antiserum D. Procedure- 1. Sterilize middle finger tip. 2. Prick the sterilized finger with a sterilized needle. 3. Collection of blood sample in vial containint 1 ml of 85% NaCl. (85% NaCl- 0.85gm NaCl dissolved in 100 ml DW) 4. Take 2 cavity slides which with 4 cavity. 5. Add a drop of 4 different sample of blood in each cavity. 6. In slides cavities add different Antiserum in sequance.

4. Observation- As per the Clumping in different cavity we can conclude the results.

5. A B D

6. Thank You