Examination Percentage in Human Blood Hemoglobin Discovery and Estimation
Hemoglobin, a vital component of blood for oxygen transport, was discovered by various scientists throughout history. Explore the process of estimating hemoglobin percentage in human blood using a haemoglobinometer and N/10 HCl. Learn about the significance of hemoglobin and its molecular structure in this practical examination session.
Download Presentation
Please find below an Image/Link to download the presentation.
The content on the website is provided AS IS for your information and personal use only. It may not be sold, licensed, or shared on other websites without obtaining consent from the author.If you encounter any issues during the download, it is possible that the publisher has removed the file from their server.
You are allowed to download the files provided on this website for personal or commercial use, subject to the condition that they are used lawfully. All files are the property of their respective owners.
The content on the website is provided AS IS for your information and personal use only. It may not be sold, licensed, or shared on other websites without obtaining consent from the author.
E N D
Presentation Transcript
Examination Percentage In Human Blood of Hemoglobin Class- B.Sc. III Year Practical Date: 24.09.2020 Time: 03:00-05:00PM Presented by Manoj Joshi Department of Zoology UCoS,MLSU, Udaipur.
Hemoglobin (1825) discovered J.F. Engelhart the hemoglobin in several species. Gilbert confirmed Engelhart in 1925. The oxygen-carrying property of hemoglobin was discovered by H nefeld in 1840. In 1959, Max Perutz and John Kendrew determined the molecular structure of hemoglobin by X-ray crystallography. The role of hemoglobin in the blood was elucidated by French physiologist Claude Bernard.
Estimation of Hemoglobin Percentage Requirements-Haemoglobinometer and N/10 HCl Procedure- 1. Sterilize middle finger tip. 2. Prick the sterilized finger with a sterilized needle and discard the 1st drop of blood. 3. Suck the blood up to 0.2 ml mark. 4. Transfer the blood in tube containing small amount of N/10 HCl. 5. Now add the N/10 HCl drop by drop by comparing with adjacent colored tube of instrument.
Observation- By continuing addition of N/10 HCl drop by drop we find the end point on which colour of both the coloured instrument tube is similar to experimental tube and now we evaluate % of Hb in given blood sample.
