Exploring Bacterial Conjugation and Microbial Genetics LT-8
Dive into the fascinating world of microbial genetics and bacterial conjugation with topics such as bacterial conjugation, replicon fusion, Hfr transfer, and interrupted mating. Explore the mechanisms of integration of F, homologous recombination, and more in this informative content.
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Presentation Transcript
Microbial genetics LT-8
Bacterial Conjugation INSERTION OF F INTO THE E. c Homologous recombination. If the recombinational event is physically reciprocal, homologous recombination between two identical IS elements, one in the chromosome and one in F, would yield flanking IS elements. Homologous recombination requires either the bacterial Rec system (described later) or a transposon gene product 2. Replicon fusion. An Hfr cell could arise by formation of a cointegrate mediated by an IS element in F, resulting in the duplication of a target sequence in the chromosome coli CHROMOSOME
Integration of F by a reciprocal exchange between an IS element in F and a homologous sequence in the bacterial chromosome
