Exploring Bio Bricks in Genetic Engineering
Discover the world of Bio Bricks, a set of interchangeable genetic parts used for creating novel gene combinations. Learn about the International Genetically Engineered Machines (iGEM) competition, the Bio Brick registry, assembly methods, prefixes, and suffixes. Dive into the intricate world of genetic manipulation and design with Bio Bricks.
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Presentation Transcript
Chapter 13 A bit about Bio Bricks
Bio Bricks 1. A set of parts that can be assembled to generate novel combinations of promoter/coding unit and other parts (terminators, ribosome binding sites etc.) 2. International Genetically Engineered Machines, IGEM, a group from MIT They sponsor a competition among students for the best design of a genetically engineered machine and they maintain a parts registry 3. They maintain a list and information about over 20,000 Bio Brick parts. The Registry contains: Information and characterisation data for all parts, device and system A catalogue which describes the function, performance and design of each part 4. Made available to use for non-profit research 5. There are different ways to assemble; we ll cover just the one used in your simulation
You can order the components you want: Examples of Bio Brick parts include promoters, ribosome binding sites (RBS), coding sequences, and terminators Every Bio Brick part has its unique identification code which makes the search for the desired Bio Brick part easier The registry is open access, so anyone can submit a Bio Brick part. Most of the Bio Brick submission is from students participating in the annual iGEM competition hosted every summer. The Registry allows exchange of data and materials online which allows rapid re-use and modifications of parts by the participating community
Prefixes and Suffixes Each part is usually in a vector with its own antibiotic resistance, ori etc. It has a prefix , set of restriction sites to the right of it and a suffix , set of restriction sites to the left of it Prefix: EcoRI, NotI, XbaI Suffix: SpeI, NotI, PstI XbaI and SpeI make compatible overhangs
EcoRI XbaI SpeI PstI EcoRI XbaI SpeI PstI GAATTC notI TCTAGA CTTAAG notI AGATCT ACTAGT notI CTCTAG TGATCA notI GAGATC GAATTC notI TCTAGA CTTAAG notI AGATCT ACTAGT notI CTCTAG TGATCA notI GAGATC EcoRI XbaI SpeI PstI CTAGA T ACTAGT notI CTCTAG TGATCA notI GAGATC GAATTC notI TCTAGA CTTAAG notI AGATCT A TGATC EcoRI XbaI SpeI PstI GAATTC notI TCTAGA CTTAAG notI AGATCT CTAGA A TGATC T ACTAGT notI CTCTAG TGATCA notI GAGATC
EcoRI XbaI SpeI PstI CTAGA A TGATC T GAATTC notI TCTAGA CTTAAG notI AGATCT ACTAGT notI CTCTAG TGATCA notI GAGATC ACTAGT notI CTCTAG TGATCA notI GAGATC GAATTC notI TCTAGA CTTAAG notI AGATCT ccdB
EcoRI XbaI SpeI PstI ACTAGT notI CTCTA TGATCA notI G AATTC notI TCTAGA G notI AGATCT CTAGA A TGATC T ACTAGT notI CTCTAG TGATCA notI GAGATC GAATTC notI TCTAGA CTTAAG notI AGATCT ccdB
XbaI EcoRI SpeI PstI ACTAGT notI CTCTA AATTC notI TCTAGA ACTAGT notI CTCTAG TGATCA notI GAGATC TGATCA notI G CTAGA GAATTC notI TCTAGA CTTAAG notI AGATCT G notI AGATCT A ccdB TGATC T
