Gel Electrophoresis and Factors Affecting Mobility
Gel electrophoresis is a technique used to separate and analyze DNA, RNA, or proteins based on their size and charge. The gel is made from agarose, a polysaccharide found in seaweed, and TAE buffer is used to conduct electricity. Factors affecting mobility include agarose concentration, particle size, conformation, charge, voltage, and time.
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Presentation Transcript
Gel Electrophoresis Gel is made from agarose Agarose is a polysaccharide found in seaweed TAE is a buffer TAE is also used to conduct electricity Wells are made to accept samples Charged sample particles migrate through the gel Dye is added for viewing samples 1
Factors Affecting Mobility Concentration of agarose in gel Size of particles Conformation of particles Charge of particles Voltage applied Time 2
