Gel Electrophoresis for DNA Fragment Separation
Gel electrophoresis is a technique used to separate DNA fragments based on size by applying an electric field through a gel medium. The process involves cutting DNA with restriction endonucleases, loading samples into wells, staining DNA, and visualizing the separated fragments. This PowerPoint presentation illustrates the step-by-step process of gel electrophoresis, from preparing the gel to running the electrical current through the chamber and staining DNA fragments with ethidium bromide.
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Presentation Transcript
Gel electrophoresis L Mathias
Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied.
DNA cut up using restriction endonucleases
Make up the gel which the DNA will be put into
DNA is stained using ethidium bromide
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