Laboratory Sample Management Protocol for Research Facilities
Explore the detailed log samples and data collection procedures for laboratory operations, including depth, replicates, pre-aliquoting, and filtration techniques. Gain insights into various equipment used and storage conditions for chemicals. Enhance your understanding of sample handling techniques in research settings.
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LOG-SAMPLES_S-LAB-OTHER E1/B2 YYYY MM DD # # # _S-LAB-OTHER LOG_SAMPLES_ _STATION- OPERATOR(S) HC HC-G Cryo-5mL LN2 #1 CP-G Cryo-5mL LN2 #1 SG FC-P FC-G Depth Replicates Cryo-5mL LN2 #1 Cryo-5mL LN2 #1 Cryo-2mL LN2 #3 Cryo-2mL LN2 #3 Z00 R01 ###-Z00 HC-1 ###-Z00 HC-G-1 ###-Z00 CP-G-1 ###-Z00 SG-1 ###-Z00 FC-P-1 ###-Z00 FC-G-1 m Z00 R02 ###-Z00 HC-2 ###-Z00 HC-G-2 ###-Z00 CP-G-2 ###-Z00 SG-2 ###-Z00 FC-P-2 ###-Z00 FC-G-2 m Z00 R03 Glutaraldehyde prealiquot at - 20 C Glycine-betaine prealiquot at 4 C ###-Z00 HC-3 ###-Z00 HC-G-3 ###-Z00 CP-G-3 PFA prealiquot at -20 C m Z00 R04 ###-Z00 HC-4 ###-Z00 HC-G-4 Glycerol prealiquot - RT m Z00 R05 DGAS DGAS DGAS ###-Z00 HC-5 ###-Z00 HC-G-5 12 mL exetainer +4 C 12 mL exetainer +4 C 12 mL exetainer +4 C m Z00 R06 ###-Z00 HC-6 ###-Z00 HC-G-6 ###-Z00 DGAS-1 ###-Z00 DGAS-4 ###-Z00 DGAS-7 m Z00 R07 ###-Z00 HC-7 ###-Z00 HC-G-7 ###-Z00 DGAS-2 ###-Z00 DGAS-5 ###-Z00 DGAS-8 m Z00 R08 ###-Z00 HC-8 ###-Z00 HC-G-8 ###-Z00 DGAS-3 ###-Z00 DGAS-6 ###-Z00 DGAS-9 m Glycerol prealiquot - RT + 100 L ZnCl2 + 100 L ZnCl2 + 100 L ZnCl2 Prealiquot No prealiquot eDNA Watera capsule RT Filtration Duration (minutes) Filtration Volume (Litres) Depth Replicate + 50 mL of buffer < 0.45 m Z00 30L 30 min. ###-Z00 eDNA => Collect filtrate for SS protocol onland : V<0.45 L min. m Z02 30L 30 min. ###-Z02 eDNA L min. m LOG-SAMPLES_S-LAB-OTHER_recto_V12012024
LOG-SAMPLES_S-LAB-OTHER E1/B2 Depth Replicate COMMENTS Z00 R01 m Z00 R02 m Z00 R03 m Z00 R04 m Z00 R05 m Z00 R06 m Z00 R07 m Z00 R08 m LOG-SAMPLES_S-LAB-OTHER_verso_V12012024
LOG-SAMPLES_S-LAB-DECKNET-5 E2 YYYY MM DD # # # LOG_SAMPLES_ _STATION- _S-LAB-DECKNET-5 OPERATOR(S) Volume filtered (Litres) Depth Z00 10 L L m Z02 10 L L m FM5-2* Falcon-15mL FRG +4 C SG5-2* Cryo-5mL LN2 FM5-1* Falcon-15mL FRG +4 C SG5-1* Cryo-5mL LN2 Depth Z00 ### Z00 SG5-1 ### Z00 SG5-2 ### Z00 FM5-1 ### Z00 FM5-2 m Z02 ### Z02 SG5-1 ### Z02 SG5-2 ### Z02 FM5-1 ### Z02 FM5-2 m *pre-aliquoted 5 mL PFA/GLUT * pre-aliquoted Glycine betaine store at -20 C store at 4 C LOG-SAMPLES_S-LAB-DECKNET-5_recto_V12012024
LOG-SAMPLES_S-LAB-DECKNET-5 E2 COMMENTS SG5 Depth Z00 R01 m Z00 R02 m COMMENTS FM5 Depth Z00 R01 m Z00 R02 m LOG-SAMPLES_S-LAB-DECKNET-5_verso_V12012024
LOG-SAMPLES_PPN2-PPN2exe E3 YYYY MM DD # # # LOG_SAMPLES_ _STATION- _PPN2-PPN2exe OPERATOR(S) 13C-DIC time (UTC) in -20 C 15N-DIC time (UTC) in gaz Depth Spikes Z00 1 : : m Z00 2 : : m Z00 3 : : m Natural abundance T0 NAT Filtered volume (mL) Filtration time start Filtration time end Filter in alu -20 C Depth Z00 ###- NAT-1 NAT-1 : : m Z00 ###- NAT-2 NAT-2 : : m Z00 ###- NAT-3 NAT-3 : : m 24h (+/- 30min) later Filtration < 40min PPN2 PPN2exe* Exetainer-12mL +4 C Filtration volume (mL) Incubated bottle Filtration time start Filtration time end Filter in alu -20 C Depth Z00 ###- PPN2-1 ###- PPN2-1 : : PPN2EXE-1 m Z00 ###- PPN2-2 ###- PPN2-2 : : PPN2EXE-2 m Z00 ###- PPN2-3 ###- : PPN2-3 : PPN2EXE-3 m ###- PPN2-BLANK * +100 L ZnCl BLANK LOG-SAMPLES_PPN2-PPN2exe_recto_V12012024
LOG-SAMPLES_PPN2-PPN2exe E3 Depth COMMENTS LOG-SAMPLES_PPN2-PPN2exe_verso_V12012024
