Lipids Experiment
This experiment aims to understand the solubility of lipids in different solvents and identify various types of lipids through tests like emulsification, acrolein, and Salkowski. The procedure involves testing lipid samples in solvents and conducting specific tests to detect lipids like cholesterol. Explore the world of lipids and their properties through practical experimentation.
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Presentation Transcript
Aim of Experiment To know solubility of lipids among different solvents and identifying those are lipids .
Type of lipids A simple lipid is a fatty acid ester of different alcohols and carries no other substance. A Complex lipid contains frequently three or more chemical identities and it has polar properties. A Derived lipids are the substances derived from simple and complex lipids by hydrolysis.
Emulsification test or Solubility test of lipids The emulsion test is a method to determine the presence of lipids using wet chemistry. The procedure is for the sample to be suspended in ethanol, allowing lipids present to dissolve. The liquid (alcohol with dissolved fat) is then decanted into water.
Acrolein test Its test used for detecting lipids in samples if they are presented by giving irritating smell . The procedure of this test is using Sodium bi-sulphate or Potassium bi-sulphate and heating the sample for a periodic time until you get the result.
Salkowski Test In this test the sample is dissolved in chloroform and an equal volume of strong sulfuric acid is added. if cholesterol is present, the solution becomes bluish red and slowly changes to a violet red, and the sulfuric acid becomes red with a green fluorescence.
Procedure Bring 5 different test tubes labling them A,B,C,D, and E . Add 2 ml of solvents individually in each labelled test tubes . Pour 3 drops of sample in each labelled test tubes . Set timer for each of them and record required time for dissolving.
Salkowski test procedure Bring a test tube includes sample in chloroform solvent . Add a few drops of concentrated sulfuric acid . Shake it well until you get the red solution that indicates having cholesterol in the sample.
Salkowski result Observation A. Before adding sulfuric acid. B. After adding sulfuric acid
