MAKING THE INVISIBLE VISIBLE
Green Fluorescent Protein (GFP) has revolutionized biological research by enabling scientists to visualize cellular processes that were once invisible. This remarkable protein, originally isolated from a species of jellyfish, emits a green glow when exposed to certain wavelengths of light. Scientists have harnessed GFP to track gene expression, monitor protein localization, and visualize dynamic interactions within cells. By making the invisible visible, GFP has opened new frontiers in the study of complex biological systems.
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Presentation Transcript
MAKING THE INVISIBLE VISIBLE GREEN FLUORESCENT PROTEIN
From gene to trait Attribution: Dhorspool at en.wikipedia
Gene Regulation http://en.wikipedia.org/wiki/File:Gene_e xpression_control.png
Proteins provide structure and function Intracellular structures Extracellular structures Signaling molecules Hormones Receptors Enzymes Gene regulators Pigments
Genetic Engineering and GFP Make a copy of the gene for GFP Modify it Attach a sequence to it that regulates a gene of interest moves a protein to a particular organelle Attach it to the gene for a known protein Insert the sequence into a cell GFP reports cellular or gene activity!
Engineering GFP itself From A Nobel for GFP, By Michael Eisen | October 8, 2008 http://www.michaeleisen.org/blog/?p=59
Mitosis By Ali Zifan - Own work; Used information from:Campbell Biology (10th Edition) by: Jane B. Reece & Steven A. Wasserman.and Nature.com., CC BY-SA 4.0, https://commons.wikimedia.org/w/index.php?curid=49721676
LLCPk cells Pig kidney epithelial cells
Phase contrast microscopy Find cells in phase microscopy Find a field of view with lots of cells Switch between eye and side Take an image. Save to desktop
Fluorescence microscopy Turn on Xenon lamp See microtubules and myosin with LP filter Microtubules with B filter Myosin with G filter Everybody look!
Taking a color image Focus carefully Take and save an image through B filter. DO NOT MOVE SLIDE OR TOUCH FOCUS KNOB Take and save another image through G filter Open both images Image/color/merge Choose correct channels Save as onto the desktop
