Master Skyline Mass Spectrometry Software with Live Reports Tutorial Webinar
"Join the tutorial webinar series to learn how to maximize the potential of Skyline mass spectrometry software, featuring live reports and expert insights. Explore Skyline Live Reports, study data processing tutorials, and detailed experiment details. Don't miss out on this valuable resource!" (310 characters)
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Presentation Transcript
Tutorial Webinar #23 Welcome to the next in the series of tutorial webinars designed to help you get the most out of Skyline mass spectrometry software. We ll begin shortly Webinar Audio: Make sure you have audio during the webinar. Click Audio Settings in the lower left-hand corner of the screen : Computer audio (preferred) through your computer s speakers or a headset Phone call (US and International numbers available) By phone, use conference ID: 961 1680 7175 **Note: we currently have music playing which you should be able to hear.** Submit Skyline Questions: the Q&A form in Zoom to submit Skyline questions to the presenters Have logistic issues? Use the Chat applet in Zoom for report webinar software (Zoom) issues or other problems.
Tutorial Webinar #23 Using Skyline Live Reports With Brendan MacLean (Principal Developer, Skyline) Nick Shulman (Lead Developer, Skyline) Mark Belanger (Project Manager, Skyline)
Agenda Welcome 250+ registered, from the Skyline team! Using Skyline Live Reports Introduction with Brendan MacLean Tutorial with Nick Shulman
Nick Shulman Microsoft Access developer Joined web database startup in 2000 Worked at LabKey Software (powers Panorama) Joined MacCoss lab in 2008 Responsible for most reports (tabular data) available from Skyline Now answers most support questions Reviews all code going into Skyline and writes a lot of it
Reports in Skyline File Export Report View Document Grid View Live Reports Document Grid Settings Document Settings - Reports
Reports in Skyline Copy Export Other Grids Check to Save With Document
Grouped Study Data Processing Tutorial Detecting plasma proteins with altered levels in a Dahl salt sensitive rat model 7 Rats on a Low Salt Diet 7 Rats on a High Salt Diet 109 proteins reported in literature as related to heart disease https://skyline.ms/tutorial_grouped.url
Experiment Details Rat plasma experiment Daniela Tomazela at University of Washington 14 subjects (7 health, 7 diseased) Technical triplicate Method design Proteins from literature No labeled reference peptide Sparse spectral library coverage Exhaustive measurement of tryptic peptides Exhaustive measurement of y3 y(n-1) product ions Refinement
Nick Shulman Tutorial Document Grid Annotations Pivoting Reports Other Reports (Other Grids)
Questions? Ask any questions at the Q&A form in Zoom: Take the post-webinar survey: https://skyline.ms/survey4webinar.url
Tutorial Webinar #23 This ends this Skyline Tutorial Webinar. Please give us feedback on the webinar at the following survey: https://skyline.ms/survey4webinar.url A recording of today s meeting will be available shortly at the Skyline website. We look forward to seeing you at a future Skyline Tutorial Webinar.
