Microbiology Experiment Results and Observations
"Explore the findings of various microbiology experiments including starch metabolism, oxygen needs, and quick gram tests. See observations, evaluations, and results for different bacteria strains from Slovakia, Poland, and Greece."
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Presentation Transcript
Presentationof group 8 Barbora Barto ov Slovakia Konrad Brodziak Poland Evangelia Petrou Greece
Observationof 1. experiment(E1) -starch metabolism A: OBSERVATION Some bacteria are visible because of thin layer of Lugolsolution. Smiley did not appear. B: OBSERVATION Smiley appeared. A B
Evalution of 1. experiment (E1) - starch metabolism A: EVALUATION Starch was not metabolised. The bacteria is not able to metabolise the starch. B: EVALUATION Starch was metabolised. The bacteria is able to metabolise the starch. A B
Result of 1. experiment (E1) - starch metabolism A: RESULT Escherichia coli B: RESULT Bacillus subtilis A B
Observation of 2. experiment (E2) oxygen need A: OBSERVATION Visible bacteria are on top of and inside the LB-agar. B: OBSERVATION Visible bacteria are only on top of LB-agar.
Evalution of 2. experiment (E2) oxygen need A: EVALUATION Bacteria A is facultative anareobe. B: EVALUATION Bacteria B is obligate aerobe.
Result of 2. experiment (E2) oxygen need A: RESULT Escherichia coli B: RESULT Bacillus subtilis
Observation of 3. experiment (E3) quick gram test A: OBSERVATION Mixture of KOH solution and bacteria A did not make the stitches. Viscosity of this mixture was greater than the mixture with bacteria B. A B: OBSERVATION Mixture of KOH solution and bacteria B did not make the stitches. Mixture have viscosity of water. B
Evalution of 3. experiment (E3) quick gram test A: EVALUATION Cell wall was broken. Gram negative bacteria. A B: EVALUATION Cell wall not was broken. Gram positive bacteria. B
Result of 3. experiment (E3) quick gram test A: RESULT Escherichia coli A B: RESULT Bacillus subtilis B
Conclusion of experiments E1 - E3 Bacteria A: Cannot metabolise starch. Facultative anareobe. Gram negative. Escherichia Escherichia coli Bacteria B: Can metabolise starch. Obligate aerobe. Gram positive. Bacillus Bacillus subtilis coli subtilis
Experiment 4 (E4) DNA isolation Sample 8.1 Human saliva Bacteria grew in pure colonies 8.1 Concentration: 90ng L 1 Sample 8.2 Human fingerprint Bacteria did not grow 12.2 Concentration: 64 ng L 1
Thanks for your attention
