Learn the essential techniques in microbiology for isolating and processing specimens, including dilution methods and specimen collection practices. Explore how to create pure cultures and perform microbiological examinations efficiently.
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Isolation Microorganisms occur in huge numbers. *Isolation of single species (pure culture) is done by : Streak plate method Spread plate method Pour plate method *Based on diluting sample out to a point where a single cell will give rise to a single colony
Specimen collection Many different examination from patients with suspected bacterial infection. Common specimens include urine, faeces, wound swabs, throat swabs,vaginal swabs,sputum,and blood. cerebrospinal fluid, pleural fluid, joint aspirates, tissue, bone and prosthetic material (e.g.line tips). specimens are sent for microbiological
What are dilutions in microbiology? Dilution is the process of making a solution weaker or less concentrated. In microbiology, serial dilutions (log dilutions) are used to decrease a bacterial concentration to a required concentration for a specific test method, or to a concentration which is easier to count when plated to an agar plate
