Microscopic Observation and Staining Techniques for Bacteria
Discover the process of microscopic observation and staining of microorganisms, focusing on size, shape, grouping, and staining reactions. Learn about the use of basic dyes to reveal internal and external structures of bacteria, as well as the preparation of fixed smears for study. Explore images depicting attraction between dyes and bacterial charges, bacterial smear preparation, simple staining, and gram staining to understand bacterial shapes.
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Presentation Transcript
Figure (5) Attraction between the positive charges of the dyes the microscopic observation of the internal and external structures of microorganisms in addition to revealing their size, shape, grouping, and staining reaction. The dyes used to stain bacteria are basic dyes. This dyes contain ionizable groups (positive charge) that are attracted to the negatively charge components of the bacterial cell. (fig.5)
Preparation of fixed smears: (fig.6) 1- When the bacteria are taken from solid media, put one drop of water on clean side, suspend the bacteria with the drop of water and spread it over the slide. 2- Allow the smear to dry in air. 3- Fix the smear to the slide by passing rapidly through a flame for 2 to 3 times. Figure (6 Bacterial smear preparation
