
Microscopy Slides and Techniques Overview
Explore an in-depth overview of microscopy slides and techniques, including Nicol, Glan-Thompson, Wollaston, Rochon, Balsam, and more. Detailed information and images provided for each slide and technique.
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Presentation Transcript
1Nicol W.Nicol, 1828 0 102 C 0 0 0 78 78 102 0 102 102 78 0 102 0 78 A 0 102 0 102 3 102 3
48641 . 1 = n e o 65836 . 1 = n o 0 68 S o 0 14 e 0 S 0 14 48 0 71 e Canada Balsam n=1.55 e
2GlanThompson o e e e i i o o
3Wollaston o e e o o e o e o e o n n o e e
2o i e 2e i 1i o 1i o e 1i 2o i 2e i n n n n = sin sin o 2e i i sini = = sin sin sin sin n n i i n n 2e i i 1 1 o 1 e e = sin sin e 2o i i e 1 o 2o sini 1 1 o n n 2o i i 2e i o e 1
4Rochon o = = sin sin sin sin n n i i n n 2o i i o 1 o o i 1 i e 2e = 2e i 2o 1 1i 1i o n n = e sin sin o 2e i i 1 e 2e i 2o i
= = L n d L n d = o o e e = ( ) n n d L L L e o e o 2 = = ( ) n n d e o 1 2 1 + = + L m m m 4 4 2 4 1 = + = + L m 2m 2 2 m 2 d = 2m = L light vector e o o e
= cos( ) E A t kz y e o 0 0 y y e y = + cos( ) E A t k z = cos( ) E A t kz 0 o y 0 0 x x y o x 0z e x = x cos( ) E A t k z 0 o x o z t0 z t
y e = cos( ) E A t kz 0 y y = cos( ) E A t y kz 0 x x y = o cos( ) E A t kz kn d x 0 e y x = cos( ) E x A t kz kn d e 0 o x o z 2 = = k E A A knd x = + cos t e o 2 x = ( ) n n d cos( ) E t o e y y
1. Babinet Wallaston = = = = , , , L n d L n d L n d L n d o1 o 1 e1 e 1 o2 o 2 e2 e 2 = + ) ( + ( ) n d n d n d n d o 1 e 2 e 1 o 2 = ( )( ) n n d d o e 1 2 1 d 2 d
Thorlabs 2.5
2Soleil Babinet = ( )( ) n n d d 1d o e 1 2 1 d 2 d 2 = ( )( ) n n d d o e 1 2