Molecular Biotechnology Course Overview and Objectives
Explore the Molecular Biotechnology course covering topics such as DNA technology, gene regulation, and applications in various fields. Learn about recombinant DNA, enzyme production, DNA manipulation techniques, and more. Develop a comprehensive understanding of gene sequencing, protein extraction, and molecular techniques with practical applications in biotechnology.
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COURSEBOOK AND INTRODUCTION Dr. Salah Dr. Salah Tofik Miss Amna Miss Amna Course: Molecular Biotechnology Course: Molecular Biotechnology MA Code408 MA Code408 Summer school: Week 1 Summer school: Week 1 Date 18/8/2024 Date 18/8/2024 Tofik Balaky Balaky
Course program: Course title: Molecular Biotechnology 4th year. Number of credits 2hrs practical per week Lecturer: Dr. Salah Tofik Balaky and Mr. Ahmed (Lab) Course length 15 weeks (course program) Teaching methods (power point presentation)
Course Overview Molecular biotechnology has largely helped scientists to understand the structure, function and regulation of genes. The development of modern Molecular biotechnology is primarily based on the success of DNA technology. Molecular Biotechnology is an interdisciplinary approach for applications to human health, agriculture, industry and environment.
Course objectives Recombinant DNA and Technology in the production of Drugs Recombinant DNA and Technology in the production of Enzymes and Vitamines. Recombinant DNA and Technology in the production of Amino acids. DNA sequencing and Transfer of genetic materials in bacteria.
Course Contents 1.Coursebook introduction, general procedure, and lab safety in DNA Manipulation. 2.DNA Extraction methods. 3.RNA Extraction Methods. 4.Restriction enzyme digestion and gel electrophoresis. 5.PCR. 6.RT-PCR.
Course Contents. 7. Midterm Exam. 8. Gene expression analysis and cDNA generation and REST Program. 9. Proteomics and Protein Extractions and SDS Page. 10. 2D SDS Page. 11. Western Blotting. 12. Enzyme production. 13. Final Exam.
Student's obligation Attendance on time is an essential requirement for all students. The engagement of every student will be monitored, as Classroom participation is important Mobile phones must be turned off.
Examinations The exams will be held during the course. Theoretical and practical exams: one per semester. Final exam Theoretical and practical Exams are composed of SCQ (single choice questions), blanks, true and false, short essays such as definitions and Diagrams
Examinations and Grading: Assessment grading (theory and practical): Exams 30% Quizzes and daily activity 30% Midterm total 60% Final exam: 40%.
References Applied Molecular Biotechnology The Next Generation of Genetic Engineering Edited by Muhammad Sarwar Khan. An Introduction to Molecular Biotechnology Fundamentals, Methods, and Applications, Edited by Michael Wink. Online Journal Articles Online Journal Articles.
Next Lecture DNA Extraction Methods
