Novel Alternatively Spliced Isoforms in CYFIP1, ZMYM6, and TRAP150 Genes
Explore the discovery of novel alternatively spliced isoforms in CYFIP1, ZMYM6, and TRAP150 genes through RT-PCR and DNA sequencing. Multiple splice variants were identified, shedding light on the intricate genetic landscape. Electropherograms showcase the novel junction sequences, providing insights into gene expression regulation. Delve into the intricate world of gene splicing and alternative isoforms in this innovative study.
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A. ZMYM6-variant missing Exon 2 B. ZMYM6-variant missing Exon 5 Supplementary Figure 2A. A G A A G G A G C A T C G A A A C T C G A Exon 1 Exon 3 Exon 4 Exon 6 C. ZMYM6-variant missing Exon 4 D. ZMYM6-variant missing Exons 4 & 5 T A T T G A G A C A T T A T T G A C T C G Exon 3 Exon 6 Exon 3 Exon 5 E. TRAP150-variant missing Exon 4 F. CYFIP1-E16 (variant 1 missing Exon 16) C A C T C A G A A A C G C T C T G A G G T A T T C T Exon 15 Exon 17 Exon 3 Exon 5
Supplementary Figure 2S.Novel alternatively spliced isoforms. A. Sequence confirmation of six novel splice junctions identified for CYFIP1, ZMYM6, and TRAP150. Multiple ZMYM6 variants were detected using a forward primer located in exon 1 and a reverse primer in exon 6. This RT-PCR assay detected five alternatively spliced variants (exon 2 missing, exon 4 missing, exons 2 & 4 missing, exons 2 & 5 missing, and exons 2 & 4 & 5 missing). Two new splice variants were also detected for the TRAP150 (missing exon 4) and CYFIP1 (missing exon 16)genes. Electropherograms show exon junction sequences representing the observed novel alternatively spliced isoforms for ZMYM6 (A-D) ZMYM6, (E) CYFIP1 (missing exon 16), and (F) TRAP150 (missing exon 4).
Suppl. Figure 2B. Probe ID Probe ID- -Exon Exon Exon Exon array array
Supplementary Figure 2S.Novel alternatively spliced isoforms. B.Alternatively spliced variants of CYFIP1, ZMYM6, and TRAP150 identified by RT-PCR and DNA sequencing. Probe set and start codon locations are labeled for the schematic representation of each gene variant. This illustration summarizes how follow up RT-PCR experiments on selected differentially expressed exons resulted in the identification of alternatively spliced isoforms.
