Oncolytic Herpes Virus Armed with CXCR4 Antagonist in Glioblastoma

Armed with a CXCR4 antagonist, oncolytic herpes simplex virus type 1 disrupts the CXCR4/CXCL12 pathway in glioblastoma, inhibiting invasion both in vitro and in vivo. Promising results show impaired invasion abilities of human and murine glioblastoma cells. The study offers a perspective on engraftment treatment and potential advancements in glioblastoma therapy.

• Oncolytic
• Herpes virus
• Glioblastoma
• CXCR4 antagonist
• Therapy

medidoc Follow

Uploaded on Feb 15, 2025 | 0 Views

Download Presentation

Please find below an Image/Link to download the presentation.

The content on the website is provided AS IS for your information and personal use only. It may not be sold, licensed, or shared on other websites without obtaining consent from the author.If you encounter any issues during the download, it is possible that the publisher has removed the file from their server.

You are allowed to download the files provided on this website for personal or commercial use, subject to the condition that they are used lawfully. All files are the property of their respective owners.

The content on the website is provided AS IS for your information and personal use only. It may not be sold, licensed, or shared on other websites without obtaining consent from the author.

Presentation Transcript

1. Oncolytic Herpes Simplex Virus type 1 armed with CXCR4-antagonist P2G to disrupt CXCR4/CXCL12 pathway in Glioblastoma Maxime Dubois1, D Arrigo Paolo1, S nchez-Gil1 Judit, Lebrun Marielle1, Lassence C dric1, Neirinckx Virginie2, Lombard Arnaud2, Aldenhoff Th r se2, Brouwers Beno t2, Rogister Bernard2 & Sadzot-Delvaux Catherine1 1Laboratory of Virology and Immunology, GIGA Infection, Inflammation and Immunity (GIGA I3), University of Li ge, 4000 Li ge, Belgium. 2Laboratory of Nervous System Disorders and Therapy, GIGA-Neurosciences, University of Li ge, 4000 Li ge, Belgium. INTRODUCTION 1

2. Glioblastoma and CXCR4/CXCL12 pathway INTRODUCTION 2

3. Oncolytic Herpes Viruses (oHSV) armed with CXCL12-antagonist (oHSV-P2G) INTRODUCTION 3

4. oHSV-P2G inhibits glioblastoma invasion in vitro Human and murine glioblastoma primary cells invasion abilities are impaired by P2G-containing conditioned media RESULTS 4

5. oHSV-P2G inhibits glioblastoma invasion in vivo Engraftment Treatment DAY 0 GB138 RFP+ Luc+ DAY 20 PBS oHSV-WT oHSV-P2G Migration volume/Whole tumour volume 1.0 0.8 ns ns 0.6 ns 1.0 0.4 ns PBS oHSV-WT oHSV-P2G 0.8 1.0 0.2 1.0 0.6 0.8 1.0 0.0 0.8 0.4 Migration model ratio 0.6 PBS oHSV-WT oHSV-P2G Migration model ratio Migration model ratio Migration manual ratio Migration manual ratio Migration manual ratio 0.8 0.6 0.2 0.4 PBS oHSV-WT oHSV-P2G 0.6 0.4 0.0 PBS oHSV-WT oHSV-P2G 0.2 0.4 Migration manual ratio Migration manual ratio Migration manual ratio PBS oHSV-WT oHSV-P2G 0.2 0.0 0.2 Migration manual ratio Migration manual ratio Migration manual ratio RESULTS 0.0 5 Migration manual ratio Migration manual ratio Migration manual ratio 0.0 Migration manual ratio Migration manual ratio Migration manual ratio

6. Conclusion Perspectives Engraftment Treatment (twice) DAY 0 GL261N4 DAY 7 + 14 PBS oHSV-WT oHSV-P2G CONCLUSION AND PERSPECTIVES 6