Original uncropped gel images
Emetine dihydrochloride inhibits invasiveness and motility of hepatocellular carcinoma cells by blocking the MAPK pathway and inducing destabilization of Twist1, as shown in original uncropped gel images. The study reveals the impact of Emetine Dihydrochloride on cellular pathways and protein expression, providing insights into potential therapeutic strategies for hepatocellular carcinoma.
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Original uncropped gel images Emetine dihydrochloride inhibits invasiveness and motility of hepatocellular carcinoma cells by blocking the MAPK pathway and inducing destabilization of Twist1 Haelim Yoon, Sayeon Cho*
Original gel images of Figure 4. The red boxes indicate the figures in the main text. Original gel images of Figure 3. The red boxes indicate the figures in the main text. A EDH (nM) B CON 50 100 200 EDH (nM) CON 50 100 200 25 Twist1 130 E-cadherin 63 100 -actin 75 48 35 130 100 E-cadherin (kDa) 75 63 Vimentin C 48 + + + MG132 (10 M) - - - 35 - 100 200 - 100 200 EDH (nM) 63 48 -actin 25 Twist1 35 (kDa) 63 -actin 48 35 (kDa)
Original gel images of Figure 5. The red boxes indicate the figures in the main text. C B EDH (nM) EDH (nM) - + - - JNKi (10uM) A CON 50 100 200 p38i (5uM) - - + - CON 50 100 200 EDH (200nM) - - - + 63 63 p-JNK 48 48 p-AKT 35 Twist1 25 35 63 63 48 JNK AKT 48 63 35 35 -actin 48 63 63 p-p38 35 48 -actin 48 35 35 63 p38 48 D FLAG-Twist1 WT 35 S68A 200 - 200 - EDH (nM) 63 p-ERK 48 35 FLAG 35 25 63 ERK 48 63 35 -actin 48 63 -actin 35 48 35 (kDa)
Original gel images of Supplementary Figure 4. The red boxes indicate the figures in the main text. Original gel images of Supplementary Figure 5. The red boxes indicate the figures in the main text. Hep3B EDH (nM) SNU886 EDH (nM) EDH (nM) CON 100 200 CON 100 200 CON 100 200 Twist 25 Twist 25 Snail1 35 63 63 25 -actin 48 -actin 48 ZEB1 135 SNU449 EDH (nM) PLC-PRF-5 EDH (nM) CON 100 200 CON 100 200 63 48 -actin Twist Twist 25 25 35 63 63 48 -actin 48 -actin 35
Original gel images of Supplementary Figure 6. The red boxes indicate the figures in the main text. Original gel images of Supplementary Figure 7. The red boxes indicate the figures in the main text. EDH (nM) D CON 100 200 SOR (10 uM) - - + + 100 EDH (100 nM) - + - + p-Stat3 75 25 Twist1 75 p-NF B 63 63 63 48 -actin -actin 48 35 35
