Oxidase Test for Bacteria Identification

This test is used to identify bacteria based on their oxidase activity. It involves a biochemical reaction that changes the color of a dye, allowing for easy differentiation of oxidase-positive and oxidase-negative organisms. The test principle, procedure, interpretation, and a list of oxidase-positive and -negative organisms are provided in detail.

• Bacteria
• Identification
• Biochemical Test
• Microbiology
• Oxidase

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1. TIU - Faculty of Science Medical Analysis Department Microbiology Heshu J. Ahmed/Assist. Lecturer _____________________________________________ Oxidase Test 2nd Stage /2nd Semester Heshu.jalal@tiu.edu.iq https://tiu.edu.iq/ 2020 - 2021

2. INTRODUCTION; It is a biochemical test Used for the identification of bacteria

3. PRINCIPLE: Cytochromes are the iron containing hemoproteins . Cytochrome system is seen in aerobic, microaerophilic and facultive anaerobic organisms.

4. IN REDUCED STATE DYE COLOURLESS IN OXIDISED STATE DYE PURPLE BLUE

5. PLATE METHOD Cultures are made on a suitable solid growth medium A freshly prepared 1% solution of tetramethyl-p- phenylene-diamine dihydrochloride (TMPDD) is poured on to the plate so as to cover the surface, and is then decanted. The colonies of oxidase positive organisms rapidly develop purple colour.

7. INTERPRETATION: Oxidase postive: purple blue colour Oxidase positive 5 to 10 sec Delayed positive 10 to 60 sec negative > 60 sec

8. OXIDASE POSITIVE ORGANISMS: Pseudomonas Neisseria Vibrio Campylobacter Aeromonas Alcaligenes Brucella Pasturella Eikenella Kingella Moraxella Legionella Helicobacter Chromobacter (oxidase variable)

9. OXIDASE NEGATIVE All enterobacteriaceae are OXIDASE NEGATIVE Acinetobacter Staphylococci streptococci

10. Thanks for your attention Heshu.jalal@tiu.edu.iq