Plant Tissue Culture Media and Supplements Overview
This content introduces plant tissue culture media types, composition, and supplements such as inorganic and organic nutrients necessary for plant growth and development in biotechnology. It covers essential elements like macronutrients, micronutrients, amino acids, vitamins, and their importance in supporting plant regeneration and nutrition.
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S. No. Content 1. Plant Tissue Culture Media 2. Types of media 3. Media composition 4. Inorganic supplements 5. Organic supplements Dr K.Kalaiarasi, Assistant Professor, Dept of Biotechnology, Padmavani Arts and Science college for women, Salem-11. 6. Other supplements 7. Preparation of media
PLANT TISSUE CULTURE MEDIA Media is a substance of supporting and provide energy and food to the developing explant. They are of following type- 1.Solid and Liquid (Suspension culture Media) depending in their appearance. 2. Depending on their concentration as well as constituents it can varies by the type of explant and species.
TYPES OF MEDIA ACCORDING TO THEIR COMPOSITION Murashige and Skoog used for plant regeneration and organogenesis White`s Medium used for root and ovule culture B5 /Gamborg Medium is a type of cell suspension used for callus culture N6/ Chu for cereal anther culture Nitsch`s used for antherculture
INORGANIC SUPPLEMENTS- Macronutrients Nitrogen Potassium Sulphur Phosphorus Magnesium Calcium
Calcium requires in cell wall formation and cell division further of it works as enzyme and enzymeactivator. Magnesium is a component of chlorophyll, enzyme activator as well as help in energyutilization. Sulphur is a part of amino acids and important for respiration of plants.
Organic Supplements Amino Acids Vitamins Antibiotics Organic Acids Energy Sources Activated Charcoal Organic Extract
Importance of Organic Supplements Vitamins are used as enzyme and its cofactors Amino acids are the constituents ofproteins Activated charcoal are used for absorbing toxin and inhibitory compounds secreted by plant Sucrose is majorlyrequired as energysource Organic extractalso contain macro and micronutrient but in unknown amount. Organic acid helpsin regeneration and development Antibiotics are usedfor maintaining contaminated free environments
Solidifying Agents Growth Regulators pH Auxin Cytokinins GibberellicAcid Abscisic Acid Agar Agarose Gel rite Clearagel Range between5.6 to 6 Not lower than 4.5 and not higherthan 7 Distil Water is used for mixing all the constituents
Preparation of Media Types of concentration 10X 100X Preparation of stock solution Addition of dry andwet contentseparately Addition of gellingagent after measuringpH Mixing of Constituents
