Practical Guide: Culture Media Preparation in Microbiology Lab
Learn the importance of culture media in microbiology experiments and the step-by-step process of preparing proper culture media. Understand the composition of different types of media and their applications in cultivating microorganisms for scientific study.
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Salahaddin University- Erbil College of Agricultural Engineering Sciences Department of Animal Resources Class: 2ndyear/1stsem. 2021-2022 Subject: Practical of Principle of Microbiology Assist. Lecturer Mr. Hemin Hussein Ali M. Sc. Biotechnology Department of Animal Resources
Third Practical Culture media preparation
Culture media preparation Aim: To prepare proper culture media. Introduction: Microorganisms require basic nutrients and certain environmental conditions to grow and reproduce. The food material on which microorganisms are grown in the laboratory is known as a culture medium (pl. media) and the growth itself is called culture. Thus culture media vary in form and composition, depending on the species to be cultivated. The ingredients of media, except the agar, are used to prepare broth or liquid media.
Culture media preparation Introduction: Agar is used to solidify liquid media, which liquefies on heating to 96 C and hardens to a jelly on cooling to 40-45 C. Medium that will achieve a desired result in the growth of organisms. Any medium that is to be suitable for a specific group of organisms must take into account the following seven factors: water, carbon, energy, nitrogen, minerals, growth factors, and pH.
On the basis of their composition, there are three main types of culture media: Natural culture media. The exact chemical composition of a natural medium is not known. e.g., Milk, urine, diluted blood, vegetable juices and meat extracts, Semi-synthetic media. The chemical composition is partially known. Any medium which contains agar. e.g., Nutrient agar, potato dextrose agar, and beef peptone. Synthetic or chemically defined culturemedia is composed of special substances of known composition. (i.e., The chemical composition is known)
There are three main forms of culture media: Liquid media (e.g., nutrient broth, citrate broth, glucose broth) Solid media (e.g., Nutrient agar and blood agar), and Semisolid media fall in between liquid and solid media. Requirements: Conical flask, Distilled water, Media components, Balance, Sterile cotton, pH paper, and Glass rod.
Culture media preparation Procedure: 100ml of distilled water was taken in a conical flask. The media (Nutrient agar and nutrient broth) were weighed The media dissolved in separate conical flask, contain 100ml d. water. Adjusted pH with the aid of NaOH and HCl ,checked by pH paper. Autoclave at 121 C for 15 minutes. (sterilized) Cooled and transferred into petri-plates and test tubes.
Quiz Write the name of three main forms of culture media with the example? Write three main types of culture media, On the basis of their composition?
