Recombinant DNA Technology and Gene Cloning
Explore the world of recombinant DNA technology, gene cloning, and the role of restriction enzymes in creating novel DNA molecules. Learn about plasmids, bacterial colonies carrying cloned DNA, gene libraries, and the fundamental techniques involved in genetic engineering.
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Presentation Transcript
Recombinant DNA and Biotechnology Recombinant DNA DNA molecules that have been joined in a test tube Gene cloning in bacterial plasmids Plasmid extrachromosomal piece of DNA not necessary for survival can be transferred between bacterial cells
Restriction enzymes are key to gene gene cloning and making recombinant DNA Site specific nucleases make double stranded cuts in DNA at their recognition sequence leave overhanging complementary ends sticky ends Two DNA molecules cut with the same restriction enzyme can be joined together.
Final joining requires DNA ligase
Vector DNA DNA from organism Recombinant DNA
Different Restriction Enzymes cut different DNA sequences
In gene cloning DNA fragments are randomly inserted into plasmids Recombinant plasmids are put into bacteria Each bacterial colony carries a different piece of cloned DNA
A gene library is a collection of cloned DNA fragments each in a bacterial colony
Do not exceed the following setting: Examples: tens 0 1 2 P 20 4 2 0 ones 1 - 20 l 1 2 0 tenths 12.2 l 4.1 l 20.0 l hundreds 0 1 2 P 200 7 3 0 tens 20 - 200 l 5 6 0 ones 136 l 75 l 200 l thousands 0 0 1 P 1000 hundreds 5 2 0 200 - 1000 l 0 5 0 tens 250 l 500 l 1000 l = 1 ml
