Reticulocyte Count in Canine and Feline Anemia
This informative content discusses reticulocyte counts in dogs and cats, including methodology, interpretation, and different types of regenerative anemia. It provides insights into the assessment of anemia severity and regenerative responses in veterinary medicine, with accompanying images for visual reference.
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Presentation Transcript
Reticulocyte Count Dry Lab Wendy Blount, DVM
The Reticulocyte Count 1. Mix EDTA blood with stain 1:1 (1:3 for birds) New methylene blue (NMB) Brilliant cresyl blue (BCB) 2. Incubate 10-20 min. for NMB, 15-30 for BCB 3. Mix blood again 4. Air dry blood smears and stain 5. Count 500-1000 RBC 6. Report % retics of RBC counted
The Reticulocyte Count Count only aggregates, not punctates Feline punctates have up to 10-15 blue dots that do not coalesce Canine punctates have 1-2 blue dots that do not coalesce
Canine Reticulocytes X X X X X RBC on the slide = 24 + 14 + 30 + 22 = 90 Reticulocytes = 20 20/90 = 22% 22% x RBC (2,400,000/ul) = 528,000/ul Markedly regenerative anemia
Canine Reticulocytes X RBC on the slide = 5 + 18 + 14 + 11 = 48 Reticulocytes = 8 8/48 = 17% 17% x RBC (1,900,000/ul) = 323,000/ul Highly regenerative anemia
Canine Reticulocytes RBC on the slide = 25 Reticulocytes = 3 3/25 = 12% 12% x RBC (2,800,000/ul) = 336,000/ul Highly regenerative anemia
Feline Reticulocytes RBC on the slide = 21 + 19 + 16 + 15 = 71 Reticulocytes = 6 6/71 = 8% 8% x RBC (2,000,000/ul) = 160,000/ul Mildly to moderately regenerative anemia
Feline Reticulocytes X X X X X RBC on the slide = 24 + 36 + 22 + 39 = 71 Reticulocytes = 7 7/71 = 9.8% 9.8% x RBC (2,800,000/ul) = 274,400/ul moderately regenerative anemia
Feline Reticulocytes X X X X X RBC on the slide = 11 + 13 + 13 + 9 = 46 Reticulocytes = 4 4/46 = 8.7% 8.7% x RBC (1,000,000/ul) = 104,400/ul mildly regenerative anemia
Acknowledgements Villers Elizabeth, Blackwood Laura BSAVA Manual of Canine and Feline Clinical Pathology, 2011 Atlas of Canine and Feline Peripheral Blood Smears Andreas Hans Hasler Textbook of Veterinary Internal Medicine, eds Stephen J Ettinger and Edward C Feldman, 6th Ed 2003
