Saccharomyces: Genus of Fungi for Food Production
Saccharomyces is a genus of fungi known for its importance in food production, including species like Saccharomyces cerevisiae used in bread, wine, and beer making. This unicellular fungus plays a vital role in various industries and has unique cellular morphology. Explore more about Saccharomyces and its diverse species in this detailed overview.
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140 Micro Lab 6 Gram stain
Purpose/Goal of Gram Stain To recognize 1. The response (Gram+ve or Gram ve) 2. Shape 3. Arrangement
It is used to differentiate between gram-positive and gram-negative bacteria, which have distinct and consistent differences in their cell walls.
The gram stain is called a differential stain because it stain cell differently based on their cell wall structure .
The material : Cultures of : Staphylococcus aureus, Bacillus subtilis, E.coli 1. Crystal violet . 2. Iodine solution . 3. Alcohol 95% . 4. Safranin . 5. Water .
Gram-positive bacteria Have a thick peptidoglycan layer surrounds the cell. The stain gets trapped into this layer and the bacteria turned purple. Gram-negative bacteria have a thin peptidoglycan layer that does not retain crystal violet stain. Instead, it has a thick lipid layer which dissolved easily upon decoulorization with Alcohol. Therefore, cells will be counterstained with safranin and turned red.
The bacteria under the microscope The bacteria under the microscope
Shape: Bacilli Arrangment: single Colour: Pink Gram s reaction: Gram s -ve Name of microorganism: E.Coli
Results: Results: shape: cocci arrangment: grape like clusters colour: violet gram s reaction: gram s +ve name of microorganism: Staphylococci
shape: Bacilli arrangment: chains colour: violet gram s reaction: gram s +ve name of microorganism: Bacillus - - (19) Gram bacterial staining - YouTube
