Scale-Up Cultures and Equipment for Bioprocessing
In bioprocessing, scaling up cell cultures involves transitioning from simple stirrer cultures to larger-scale fermentors. Different apparatus is required to handle varying cell capacities, ranging from laboratory-scale fermentors to semi-industrial pilot plants. Techniques such as monolayer cultures, suspensions, continuous cultures, air-lift fermentors, roller cultures, and fixed bed reactors play crucial roles in the scale-up process, ensuring optimal growth conditions. Gas exchange, agitation methods, and equipment scalability are key considerations to achieve successful bioprocessing at different scales.
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Presentation Transcript
Scale up *1 109 1 1010cells -- produced in simple stirrer cultures of around 1-10-L capacity *larger-scale cultures of 1 1011 cells will require apparatus ranging from a 100-L laboratory scale fermentor to a semi-industrial pilot plant with capacities of from 100 to 1000 L. 1 1012 Monolayer- increased growth surface area
Scale Up- Suspensions Increase in Volume Agitation of the medium - depth exceeds 5 mm, Above 5 10 cm, sparging with CO2 and air is required to maintain adequate gas exchange. Stirring of such cultures is best done slowly with a magnet encased in a glass pendulum or with a large-surface area paddle .
Scale-up in Monolayer cultures
