Simplifying Monocyte Isolation: How PluriBead Enhances Precision in Cell Separat
PluriBeadu2019s design eliminates the need for centrifugation and magnetic equipment, making it highly accessible for labs with limited resources. Its reliance on a simple particle filtration mechanism ensures accuracy and reproducibility while main
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Presentation Transcript
Simplifying Monocyte Isolation: How PluriBead Enhances Precision in Cell Separation Efficient cell separation is crucial for isolating specific cell types like monocytes, especially in research and clinical applications. Traditional methods often involve multiple steps, expensive equipment, or risk damaging fragile cells. PluriBead revolutionizes this process by offering a magnetic-free, straightforward, and gentle approach to isolating target cells. This article explores how PluriBead simplifies monocyte isolation while enhancing precision in particle separation techniques.
What Is PluriBead Technology? PluriBead is an innovative cell separation tool designed to isolate target cells without the need for complex equipment or extensive sample preparation. It uses antibody- coated beads to capture specific cells, which are then separated through a simple sieving process using a strainer cascade. The unwanted cells pass through the sieve, while the desired cells remain attached to the pluriBeads for further detachment and collection.
Key Advantages: No Sample Preparation: PluriBead eliminates the need for gradient centrifugation or erythrolysis. Versatility: It can process various sample materials, including whole blood, buffy coat, and tissue homogenates. Rapid Results: Isolation can begin in as little as 5 minutes. Gentle Process: Ensures high viability and yield, reducing the risk of damaging cells.
How Does PluriBead Work? Step 1: Mixing PluriBeads, coated with target-specific antibodies, are added to the sample material. This mixture is gently incubated, allowing the target cells to bind to the beads. Step 2: Separation Using a Strainer Cascade The mixture is passed through a pluriStrainer cell sieve. While the unwanted cells run through, the bead-bound target cells remain on top. This process is efficient and leverages particle filtration to achieve high precision. Step 3: Detachment Target cells are detached from the beads using a detachment buffer. The detached cells are then washed into a fresh collection tube, leaving the beads behind.
Specialized Features of PluriBead Cell Enrichment: PluriBead isolates cells with high purity, ensuring that only the desired cell types are collected. Parallel Cell Isolation: With the pluriBead cascade, researchers can separate two distinct cell types from a single sample. Sequential Isolation: Up to six different cell targets can be separated sequentially from one sample, making it a versatile solution for complex research needs. Multiple Applications: Beyond cell isolation, PluriBead can be used for viruses, bacteria, proteins, and RNA/DNA enrichment.
Choosing the Right PluriBead Size PluriBead is available in two bead sizes: S-PluriBead: Ideal for isolating small numbers of target cells from large volumes, such as circulating tumor cells (CTCs). M-PluriBead: Suited for larger target cell populations in smaller sample volumes, such as buffy coat preparations.
Why PluriBead Excels in Particle Separation Techniques PluriBead s design eliminates the need for centrifugation and magnetic equipment, making it highly accessible for labs with limited resources. Its reliance on a simple particle filtration mechanism ensures accuracy and reproducibility while maintaining the integrity of fragile cells.
Conclusion PluriBead s straightforward workflow, versatility, and ability to achieve high-purity results make it a game-changer in cell separation. Whether isolating monocytes from whole blood or enriching specific cell types for advanced studies, PluriBead delivers precision and efficiency. In conclusion, cell separation technology using PluriBead is a cost-effective, reliable, and user-friendly method for researchers and businesses seeking high-quality cell enrichment. Its flexibility across applications and species makes it a valuable tool for advancing cell-based research and therapeutic development.
