Soxhlet Extractor: Laboratory Apparatus for Lipid Extraction
Learn about the Soxhlet extractor, a laboratory apparatus invented in 1879 by Franz von Soxhlet for lipid extraction. Discover its applications, working principle, and the Soxhlet method for determining crude fat content in plant and animal tissues.
Download Presentation
Please find below an Image/Link to download the presentation.
The content on the website is provided AS IS for your information and personal use only. It may not be sold, licensed, or shared on other websites without obtaining consent from the author. If you encounter any issues during the download, it is possible that the publisher has removed the file from their server.
You are allowed to download the files provided on this website for personal or commercial use, subject to the condition that they are used lawfully. All files are the property of their respective owners.
The content on the website is provided AS IS for your information and personal use only. It may not be sold, licensed, or shared on other websites without obtaining consent from the author.
E N D
Presentation Transcript
1 A Soxhlet extractor is a piece of laboratory apparatus invented in 1879 by Franz von Soxhlet. It was originally designed for the extraction of a lipid from a solid material. This experiment so uses a soxhlet method to determine the lipid contents of plant & animal tissues.
Soxhlet Extractor A piece of laboratory apparatusinvented in 1879 by Franz von Soxhlet. Originally designed for the extraction of a lipid from a solid material. However, a Soxhlet extractor is not limited to the extraction of lipids. Typically, a Soxhlet extraction is only required where the desired compound has a limited solubility in a solvent, and the impurity is insoluble in that solvent.
3 The Soxhlet method for determining crude fat content is a lengthy process requiring up to a day for a single analysis. The solvent extraction step alone takes six hours. The method is therefore not favoured for routine testing purposes in the meat industry, rather it is used as a standard reference method.
4 In principle, fat is extracted, semi continuously, with an organic solvent. Solvent is heated and volatized then is condensed above the sample. Solvent drips onto the sample and soaks it to extract the fat. At 15-20 min interval, the solvent is siphoned to the heating flask, to start the process again. Fat content is measured by weight loss of sample or weight of fat removed.
OBJECTIVE 5 1. To extract fat from various plant & animal tissues by an exhaustive extraction using a Soxhlet extractor 2. To determine percent of fat content in a food sample by weight in a sample. 3. To study the function of extraction process by Soxhlet extractor
Schematic diagram of a Soxhlet extractor. 6 1: granules 2: Still pot (extraction pot) - still pot should not be overfilled and the volume of solvent in the still pot should be 3 to 4 times the volume of the soxhlet chamber. 3: Distillation path 4: Soxhlet Thimble 5: Extraction solid (residue solid) 6: Syphon arm inlet 7: Syphon arm outlet 8: Expansion adapter 9: Condenser 10: Cooling water in 11: Cooling water out Stirrer bar/anti-bumping
THE SOXHLET EXTRACTOR Continuous extraction of a component from a solid mixture. Boiling solvent vapors rise up through the larger side-arm 7
8 Condensed drops of solvent fall into the porous cup, dissolving out the desired component from a solid mixture
9 When the smaller side-arm fills to overflowing, it initiates a siphoning action.
10 The solvent, containing the dissolved component, is siphoned into the boiler below
11 Residual solvent then drains out of the porous cup, as fresh solvent drops continue to fall into the porous cup.
12 . . . and the cycle repeats . . .
13 It should look something like THIS: (only much SLOWER) This program will re-start in one minute. To terminate, press Esc
15 Advantage: - Isolate desired oil when it has limited solubility in a solvent and when impurity is insoluble in a solvent Disadvantages: -Long time process -Poor extraction of Polar lipids -Large volume of solvents required
