TargetP: Predicting N-terminal Presequences
"Explore the uses and benefits of TargetP, a widely used server for predicting N-terminal presequences and cleavage sites in protein sequences. Learn about the analysis parameters, input/output process, and how TargetP aids in predicting signal peptides and transit peptides for organelle targeting."
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Presentation Transcript
TargetP TargetP
Content Content Introduction to TargetP Uses and benefits of TargetP TargetP Analyzes Input & OutPut Results Analysis Parameters Conclusion
Introductio Introductio n n TargetP server predicts the presence of N-terminal presequences: signal peptide (SP), mitochondrial transit peptide (mTP), chloroplast transit peptide (cTP) or thylakoid luminal transit peptide (luTP). For the sequences predicted to contain an N-terminal presequence a potential cleavage site is also predicted.
TargetP is most widely used server in research for determination of N- terminal pre-sequences and potential cleavage site for the protein sequences.
Analyzes Analyzes Chloroplast Chloroplast transit peptide transit peptide Transit peptide is required for their transport across the relevant membranes from their site of synthesis in the cytoplasm. Mitochondrial Mitochondrial Transit Peptide Transit Peptide Small peptides that are required by the cell to transport mitochondria localizing proteins. Thylakoid luminal Thylakoid luminal transit peptide transit peptide Proteins targeted to the thylakoid lumen have bipartite transit peptides.
Input & Output Input & Output Input Input Output Output TargetP requires a single sequence (just the amino acids) or a number of sequences in FASTA format as input. TargetP predicts possible N-terminal presequences along with the potential cleavage site for the protein.
GLR1_DROME used as query to predict the N- terminal presequences using TargetP server.
Parameters Parameters Organism Organism Group Group Choose Plant for any organism with chloroplasts/plastids and Non-plant otherwise. 1st Long-Shows one plot and one summary per sequence. Short-Convenient if you submit lots of sequences. Shows only one line of output per sequence and no graphics. Output Output Format Format 2nd
Conclusion Conclusion TargetP is a widely used server for the prediction of the presence of the N- terminal pre-sequences. TargetP server can also predict a potential cleavage site for the protein sequences predicted to contain an N-terminal presequence. TargetP-2.0 is available as a stand-alone software package with the same functionality.
