Understanding Inteins: Insights into Protein Chemistry
Explore the fascinating world of inteins, self-splicing domains, and homing endonucleases in this comprehensive review session. Learn about the co-evolution of genes, mutualism, parasitism, and commensalism in intein systems. Dive into examples like split inteins and functional replicative DNA polymerase III. Discover how nature's gift to protein chemists, inteins, offers unique insights into molecular biology and biochemistry.
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Presentation Transcript
MCB 3421 2021 Class 26 Review Session
Reminders Let me know by email (gogarten@uconn.edu), if you will take the exam at the Center for Students with Disabilities Your notebooks are due before the final on Monday Be Nice ! Do the SETs complete Take-Home Exam #8 before Friday 10am Columbia from https://en.wikipedia.org/wiki/Uncle_Sam
Homing and Homing Endonucleases in Inteins Self Splicing Domain Homing Endonuclease Domain Homing Endonuclease Recognition Site
In case of inteins the co-evolution of genes results in Mutualism between splicing and homing endonuclease domain Commensalism between host protein and intein without HE domain Parasitism between host protein and intein with HE Nat.Struct.Biol. 9: 764-770 Structure from Moure et al. (2002) Encoding 1LWS Extein = Host Protein Intein Splicing Domain Homing Endonuclease (HE) domain
From: Inteins: nature's gift to protein chemists Neel H. Shah and Tom W. Muir DOI: 10.1039/C3SC52951G
Split inteins as an example for CNE Functional replicative DNA polymerase III Transcribed and translated dnaE cis splicing dnaE (difficult to revert, because no complete cDNA present) Non-covalent inter- actions between N and C intein dnaE1 trans splicing dnaE2 extein Gene encoding extein Intein splicing domains Gene encoding splicing domains Intein homing endonuclease domains Gene encoding homing endonuclease domain
From: Inteins: nature's gift to protein chemists Neel H. Shah and Tom W. Muir DOI: 10.1039/C3SC52951G
