Understanding the Importance of Wet Mount Slides in Biology Labs
Learn how to prepare and examine wet mount slides for microscopic analysis in biology labs. Discover why wet mount slides are essential for observing cellular structures, including the nucleus, cytoplasm, and cell membrane. Enhance your understanding of how water supports samples and facilitates light transmission. Start your journey into the microscopic world with this comprehensive guide.
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Presentation Transcript
Medical Biology Medical Biology Year 1 Year 1 Lab 3: Wet mount slide Lab 3: Wet mount slide By Lecturer: Lecturer: Dr. Department: Basic Sciences College of Dentistry University of Basrah Dr. Hanaa Ali Hussein Hanaa Ali Hussein University of Basrah College of Dentistry Basic Sciences
Preparing a wet mount slide Materials:- 1- Clean slide 2- Cover slip 3- Sticker 4- Methylene blue stain 5- Distilled water 6- Rack 7- Microscope University of Basrah College of Dentistry Basic Sciences 2
Preparing a wet mount slide Method:- 3- Put one drop of methyline blue or crystal violet on the slide where you have spread the cells and wait for 3 minutes. 1- Take a piece of tissue from the epithelium of the cheek or pallet by using sterilized sticker. 2- Swab the scrubbed tissue on empty slide . University of Basrah College of Dentistry Basic Sciences 3
Preparing a wet mount slide Nucleus Cell Cytoplasm Cell membrane 4- Wash up the stain using a distilled water. 6- Examine under light microscope 5- Slowly lower the upper edge of the cover slip onto the specimen, the excess water should be absorbed with paper. University of Basrah College of Dentistry Basic Sciences 4
Why we prepare a wet mount slide? The water helps support the sample and it fills the space between the cover slip and the slide allowing light to pass easy through the slide, the sample, and the cover slip. University of Basrah College of Dentistry Basic Sciences 5
