Understanding the SARS-CoV-2 Virion Structure and Life Cycle
Explore the intricate details of the SARS-CoV-2 virion structure, spike protein interactions, viral RNA genome compartmentalization, and major stages of the virus life cycle. Learn about mutations in various VOCs and the attachment and entry process into host cells. Delve into the complexity of viral replication, transcription, and assembly.
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Presentation Transcript
SARS-CoV-2 Virion Marko Bajic 2021
SARS-CoV-2 Structure and Composition Spike Protein (S) Binds to cellular receptors and inserts into the host cell membrane. S Interacts with other viral proteins and helps with virus morphogenesis, assembly, and virus budding. M Membrane Protein (M) Important for multiple aspects of a virus s life cycle. Interacts with other CoV proteins and host cell proteins. Envelope Protein (E) E Stolen from host cell s endoplasmic reticulum (ER). Wraps the virion. Lipid Bilayer Membrane Packages the viral RNA genome into a ribonucleoprotein (RNP) complex called the capsid Nucleocapsid Protein (N) N 30,000 bases of a positive-sense, single-stranded RNA genome (+ssRNA). Contains information for replication and assembly. RNA
Viral RNA Genome Compartmentalization Replicase Genome Structural and Accessory Proteins ORF1a ORF1b S 5 UTR ORF3a E M ORF6 ORF7a ORF7b ORF8 Replication Machinery cDNA RNA N ORF10 3 UTR
Genome, showing some mutations for 3 VOCs Nextstrain Clade Pango Lineage 20I/501Y.V1 B.1.1.7 20H/501Y.V2 B.1.351 20J/501Y.V3 B.1.1.28.1 (P.1) ORF1b:E5665D ORF1a:SGF 3675-3677 deletion S:E484L S:N501Y ORF8:28269-73 Insertion
Attachment and Entry 1) Receptor Binding 2) S protein Cleavage 3) Fusion and Entry RBD on S TMPRSS2 ACE2 Host Cell
Major Stages of SARS-CoV-2 Life Cycle Attachment and Entry Replicase Protein Expression Replication and Transcription Assembly and Release -3 +5 -3 +5 3CL pro RdRP Hel Translation Translation 1a and 1b Transcription N SE M Others Incorporation at ER Translation
