Voltage-to-Frequency Conversion in Analytical Instruments
Using a voltage-to-frequency converter, the detector signal is transformed into a pulse rate proportional to the input signal, aiding in peak measurement. Efficiency for column separation is calculated, making it easier to measure in terms of baseline width. The unique characteristics of compounds, detectable under UV light, and the detection of radioactive compounds on chromatograms enrich analytical research.
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Presentation Transcript
derived by feeding the detector signal into a voltage-to-frequency converter which produces a pulse rate proportional to the input signal. The total number of pulse is a measure of the peak, and this can be printed out directly or stored until required.
Efficiency, N for column separation is related to tRas follows:
It is also easier to measure in terms of base line width
W= peak width measured at half of peak height.
longer (visible) wavelength. These compounds, although invisible on chromatogram in ordinary light, can readily be detected under UV lamp. The wavelength of the light emitted and hence the colour when seen is a characteristic of the compound and is therefore useful for the purpose of identification.
nuclear power labeled radioactive compounds for research. This can be detected on chromatograms by means of special counter (Geiger Muller counter and Scintillation counter).
