Osmotic Pressure in Biology
Learn about osmotic pressure and osmolar concentration in biological systems, illustrated through the movement of water across permeable membranes due to differences in solute concentrations. Discover the concept of osmosis and how it influences cellular integrity in isotonic, hypertonic, and hypotonic solutions.
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Presentation Transcript
Lab 4 Lab 4 Osmotic pressure The Osmolar concentration mainly used in biology to explain the osmotic effect, We suggest the existence of a container divided by water permeable membrane to two sides A and B . water presence in both sides and glucose (1 M) in side B only
Osmolar Solution So to reaching equilibrate stage the water will move from (A to B) { from high water concentration to low water concentration } . This movement of water called Osmosis , and the power that mated on the membrane called Osmotic pressure
Osmolar Solution H2O 1M Glucose 1 Osm B A
Osmolar Solution Osmol (Osm) :-A unite used to explain the capability of a solution to make Osmotic pressure. Every (1 m) of glucose sheds osmotic pressure on membrane about (1 Osm) this applies to all non ionic molecules.So if we put (2M) of glucose in the side B this will cause in water movement from A side to B side causing in Osmotic pressure.
Osmolar Solution About the ionic molecules such as Nacl , when we dissolve Nacl in water Nacl molecules will break down to 2 ions . Nacl ------------> 1 Na + 1 cl 1mole ---------> 1mol + 1mole = 2mole Each ion will cause osmotic pressure of it s on , so the total osmotic pressure equal to the summation of the osmotic pressure of all the ions in the solution.
Osmolar Solution H2O (1 Na + 1 cl) 2M 2 Osm B A
Osmolar Solution Osmotic pressure of all mammals cell is 0.3 Osm {300 mOsm} milliosmolar , if we put normal cell in solution of 0.3 Osm of glucose . Or 0.15 Osm of Nacl This cell will be intact , this solution called isotonic solution.
Osmolar Solution I. Isotonic Solution :- A solution with osmotic pressure equal to osmotic pressure inside mammals cells. {the cell remain intact} II. Hypertonic Solution :- A solution with osmotic pressure higher than the osmotic pressure inside the cell. {the cell will be Shrinks } III. Hypotonic Solution :- A solution with osmotic pressure lower than the osmotic pressure inside the cell .{the cell will be destroyed }
Osmolar Solution Ex } prepare 1 Osm solution of Nacl ,not that mw of Nacl is 58.5 . Nacl ------------> 1 Na + 1 cl 58.5 2 = 29.5 gm /1 L Ex} prepare 1 Osm of Cacl2 solution , mw 110. Cacl2 --------- > 1 Ca + 2 cl 110 3 = 36.6 gm /1 L Ex } prepare 1 Osm of glucose ,mw 180.
