Cloning with Restriction Enzymes and Ligation

Learn about restriction enzyme cloning, plasmid ligation, blue-white selection, DNA recombination, and the role of ligases in joining DNA fragments. Discover the misconception about ligase activity and the adenylation process involved in DNA ligase function.

• Cloning
• Restriction Enzymes
• DNA Recombination
• Plasmid Ligation
• Ligases

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Uploaded on Mar 15, 2025 | 0 Views

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Presentation Transcript

1. Chemical Ligation Ligation: The state of being bound.

5. Commercially Available

16. Fluorenylmethoxycarbonyl chloride (Fmoc-Cl)

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